-
Plasmid#212146PurposePlasmid expressing green fluorescent protein under constitutive promoter PgyrA control.DepositorInsertgreen fluorescent protein
UseTagsExpressionBacterialMutationPromoterPgyrAAvailable sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp
Plasmid#212144PurposePreparatory plasmid for operon integration into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
INT_GFPsp_CamRcargo
Plasmid#212143PurposePreparatory plasmid for gene disruption into the genome using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC, green fluorescent protein
UseTagsExpressionBacterialMutationPromoterpJ23119Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_NEMF_CDKL1
Plasmid#205867PurposeExpress mEGFP-tagged fusion protein, NEMF_CDKL1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_CTNNB1_ULK4
Plasmid#205799PurposeExpress mEGFP-tagged fusion protein, CTNNB1_ULK4 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I432DPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_W421AD422A_eGFP
Plasmid#208880PurposeExpress eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutations W421A and D422APromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2T_eGFP
Plasmid#208883PurposeExpress eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations L191T and A225TPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_2E_eGFP
Plasmid#208884PurposeExpress eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.DepositorArticleInsertTLNRD1 (TLNRD1 Human)
UseTagseGFPExpressionMammalianMutationmutations K192E and R233EPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TLNRD1_5H_eGFP
Plasmid#208861PurposeExpress eGFP-TLNRD1 5 helix bundle (TLNRD1 lacking amino acids 142-273) in mammalian cells.DepositorArticleInsertTLNRD1 5 helix bundle (TLNRD1 Human)
UseTagsExpressionMammalianMutationDeleted amino acid 142-273PromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_wt_eGFP
Plasmid#208876PurposeExpress eGFP-CCM2 in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationWT sequencePromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I428S_eGFP
Plasmid#208878PurposeExpress eGFP-CCM2 with the I428S mutation in mammalian cells.DepositorArticleInsertCCM2 (CCM2 Human)
UseTagseGFPExpressionMammalianMutationmutation I428SPromoterAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_C99_sfGFP
Plasmid#207802PurposeYeast 2µ vector for expression of Suc2SP-C99-sfGFP-GAL4DepositorInsertSuc2SP-C100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch3_sfGFP
Plasmid#207864PurposeYeast 2µ vector for expression of Suc2SP-Notch3_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch3_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYea5.1_Notch1_sfGFP
Plasmid#207801PurposeYeast 2µ vector for expression of Suc2SP-Notch1_100-sfGFP-GAL4DepositorInsertSuc2SP-Notch1_100-sfGFP-GAL4 fusion protein
UseTagsStrep-TagIIExpressionBacterial and YeastMutationPromoterTEFAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
mhc2dab:GFP-LT
Plasmid#68996Purposefor making transgenic fishDepositorInsertmhc2dab (mhc2dab Zebrafish)
UseZebrafish expressionTagsGFP-LTExpressionMutationPromotermhc2dabAvailable sinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJ016_MTB_CoChR_KGC_eGFP_ER2
Plasmid#209277PurposeUbiquitously expresses CoChR in zebrafish via tol2 transgenesis or in vitro transcription of mRNADepositorInsertCoChR_KGC_eGFP_ER2
UseZebrafish expression, in vitro transcriptionTagsExpressionMutationPromoteractb2Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBJ035_MTB_gtACR2_KGC_eGFP_ER2
Plasmid#209278PurposeUbiquitously expresses gtACR2 in zebrafish via tol2 transgenesis or in vitro transcription of mRNADepositorInsertgtACR2_KGC_eGFP_ER2
UseZebrafish expression, in vitro transcriptionTagsExpressionMutationPromoteractb2Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_ZKSCAN5_SMO
Plasmid#205960PurposeExpress mEGFP-tagged fusion protein, ZKSCAN5_SMO from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_PICK1_POLR2F
Plasmid#205894PurposeExpress mEGFP-tagged fusion protein, PICK1_POLR2F from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only