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pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

Plasmid

#166433

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM2 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM2 CBS-pair domain

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac, LacI, Amp

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

Plasmid

#166434

Purpose

Express pGEX-6P-1 Caenorhabditis elegans CNNM5 CBS-pair domain

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

Caenorhabditis elegans CNNM5 CBS-pair domain

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Tac, LacI, Amp

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Plasmid

#166416

Purpose

Express pET-15b(+) Caenorhabditis elegans PRL (Δprenylation motif)

Depositor

Kalle Gehring

Article

Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

Insert

caenorhabditis elegans PRL

Use

Tags

N-terminal His-tag

Expression

Bacterial

Mutation

Deleted nucleotides coding for 4 C-terminal amino…

Promoter

AmpR, lac1

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Use

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS640

Plasmid

#188892

Purpose

ttTi5605 MosSci targeting vector with Pmex-5::Cas9 and floxed Cbr-unc-119 marker

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

Pmex-5::Cas9, Cbr-unc-119

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS716

Plasmid

#188888

Purpose

Targeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker and Phsp16.41::Cre

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

floxed Cbr-unc-119 marker and Phsp16.41::Cre

Use

Tags

Expression

Worm

Mutation

C. elegans codom optimization and intron addition

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS1272

Plasmid

#188891

Purpose

Ric-4 sgRNA expression vector

Depositor

Erik Jorgensen

Article

Schwartz et al PLoS Genet. 2021 Nov 8;17(11):e1009755.

Insert

PU6(R07E5.16)::Ric-4 sgRNA (CELE_Y22F5A.3 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSMNot_sra-6::trp-4

Plasmid

#182261

Purpose

trp-4 cDNA cloned into pSMNot_sra-6 at Kpn&Notl

Depositor

Sreekanth Chalasani

Article

Ibsen et al Nat Commun. 2015 Sep 15;6:8264. doi: 10.

Inserts

trp-4 (trp-4 Nematode)
sra-6 (sra-6 Nematode)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pIN2_mcm-3_mScarlet

Plasmid

#182933

Purpose

Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic mcm-3 fused to mScarlet

Use

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Mutation

Promoter

mcm-3

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_93-127 (pBS0735)

Plasmid

#185231

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_93-127. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_93-127

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q7JLY3_CAEEL_48-124 (pBS0738)

Plasmid

#185232

Purpose

For the mammalian expression of the C. elegans protein Q7JLY3_CAEEL_48-124. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q7JLY3_CAEEL_48-124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pST_Q19228_CAEEL_51-88 (pBS0734)

Plasmid

#185230

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-88. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-88

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_Q19228_CAEEL_51-127_noLink (pBS0733)

Plasmid

#185229

Purpose

For the mammalian expression of the C. elegans protein Q19228_CAEEL_51-127_noLink. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

Q19228_CAEEL_51-127_noLink

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H2FLK5_CAEEL_199-435 (pBS0696)

Plasmid

#185215

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_199-435. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_199-435

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H2FLK5_CAEEL_542-800 (pBS0695)

Plasmid

#185214

Purpose

For the mammalian expression of the C. elegans protein H2FLK5_CAEEL_542-800. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK5_CAEEL_542-800

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_A0A061ACN9_CAEEL (pBS0349)

Plasmid

#185187

Purpose

For the mammalian expression of the C. elegans protein A0A061ACN9_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A061ACN9_CAEEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H9G2U1_CAEEL (pBS0346)

Plasmid

#185185

Purpose

For the mammalian expression of the C. elegans protein H9G2U1_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H9G2U1_CAEEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H9G2T9_CAEEL (pBS0344)

Plasmid

#185183

Purpose

For the mammalian expression of the C. elegans protein H9G2T9_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H9G2T9_CAEEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H9G2T8_CAEEL (pBS0342)

Plasmid

#185182

Purpose

For the mammalian expression of the C. elegans protein H9G2T8_CAEEL. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H9G2T8_CAEEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_H2FLK4_CAEEL (pBS0314)

Plasmid

#185171

Purpose

For the mammalian expression of the C. elegans protein H2FLK4_CAEEL. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

H2FLK4_CAEEL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge