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pExT
Plasmid#36889DepositorInsertsT (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Hygro
UseTagsExpressionMammalianMutationdeleted nucleotides before nucleotide 275 and ins…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pExG
Plasmid#36888DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Hygro
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBIND-HBSP
Plasmid#53113PurposeExpression of GAL4BD-tagged Hepatitis B splicing proteinDepositorInsertHBSP
UseTagsGAL4BDExpressionMammalianMutationPromoterCMVAvailable sinceMay 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf R401H
Plasmid#118334PurposeExpression of human CrafDepositorInsertCraf R401H (RAF1 Human)
UseTagsFlagExpressionMammalianMutationR401HPromoterCMVAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 1-493
Plasmid#78581PurposeExpression flag-USP19 1-493 in mammalian cellsDepositorInsertflag-USP19 1-493 (USP19 Human)
UseTagsflagExpressionMammalianMutationmutated 494 residue to stop codonPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 494-1318
Plasmid#78587PurposeExpression flag-USP19 494-1318 in mammalian cellsDepositorInsertflag-USP19 494-1318 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 KD ∆24-mCherry
Plasmid#80272Purposemammalian expression plasmid for kinase dead Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGFP-Ub-VPS13D_dN
Plasmid#176465Purposeexpress VPS13D lacking N-terminal 117 aa in mammalian cellsDepositorInsertVPS13D (VPS13D Human)
UseTagsGFP-UbExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLPC-N-Myc-Flag-BirA-hPOT1
Plasmid#166409Purposeexpress BirA-hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsBirA, flag, and mycExpressionMammalianMutationPromotercmvAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 494-1290
Plasmid#78580PurposeExpression flag-USP19 494-1290 in mammalian cellsDepositorInsertflag-USP19 494-1290 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493 and mutated 1291 residue…PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Flag-Craf SSDD
Plasmid#118337PurposeExpression of human CrafDepositorInsertCraf SSDD (RAF1 Human)
UseTagsFlagExpressionMammalianMutationSSDDPromoterCMVAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
vsv-Sgo1 N61I-GFP
Plasmid#108497Purposeexpression of vsv-Sgo1 N61I-EGFP (no PP2A binding)DepositorInsertShugoshin 1 (SGO1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationno PP2A binding N61IPromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIDAF
Plasmid#213710PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIDAF under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR/eCas9-GPIBY55
Plasmid#213709PurposeTo express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.DepositorInsertEGFP
UseCRISPRTags3XFLAG and Twin-strep-tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT I261A
Plasmid#153476PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT I261ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, I261APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-TAPBPR-tapasinCT S333A
Plasmid#153477PurposeMammalian expression of FLAG-tagged TAPBPR with tapasin CT S333ADepositorInsertTAPBPR (TAPBPL Human)
UseTagsFLAGExpressionMammalianMutationC terminal tail of tapasin, S333APromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta2step
Plasmid#153479PurposeMammalian expression of FLAG-tagged Tapasin delta2stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.13-14, 16-17PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin delta3step
Plasmid#153480PurposeMammalian expression of FLAG-tagged Tapasin delta3stepDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationdeletion of aa.12-14, 16-18PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-Tapasin G15E
Plasmid#153481PurposeMammalian expression of FLAG-tagged Tapasin G15EDepositorInsertTAPBP (TAPBP Human)
UseTagsFLAGExpressionMammalianMutationG15EPromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only
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