Addgene

Showing: 6721 - 6730 of 152602 results

Results per page:

Sort results by:

pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

Plasmid

#174896

Purpose

Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

Mma PylRS (pylS Methanosarcina mazei)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1

Available since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-D12L

Plasmid

#89161

Purpose

Express vaccinia virus D12L in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Vaccinia virus D12L

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promotor

Available since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF3018

Plasmid

#144494

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NEUROG3 (NEUROG3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FKBP-EGFP-HOTag3

Plasmid

#106924

Purpose

Expresses FKBP-EGFP-HOTag3 in mammalian cells

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

FKBP-EGFP-HOTag3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
prDNA spacer-LacOx50-20xMS2-Hyg

Plasmid

#210219

Purpose

Integrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Inserts

50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF0949

Plasmid

#144362

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

EMX1 (EMX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
RTB300

Plasmid

#80410

Purpose

human cTNT minigene with genomic segment containing alternative exon 5

Depositor

Thomas Cooper

Article

Philips et al Science. 1998 May 1;280(5364):737-41.

Insert

TNNT2 (TNNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc

Depositor

Kai Johnsson

Article

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-Puro-TDP-43-A315T

Plasmid

#133755

Purpose

Lentiviral expression of human TDP-43 A315T disease mutant in mammalian cells.

Depositor

Shawn Ferguson

Article

Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

Insert

TARDBP (TARDBP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Changed alanine 315 to threonine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNL4-3 envFS eGFP Δvif/vpr

Plasmid

#101344

Purpose

Deletion from NL4-3 nt 5582-6199 encompassing Vif and Vpr coding sequence

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta vif/Vpr

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge