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Plasmid#200191PurposeA TETon activator, which combined with the DiLiCre 2.0 plasmid, drives the expression of DiLiCre 2.0 upon doxycycline. DiLiCre 2.0 is a cre recombinase that is activated by violet light.DepositorInsertblasticidine - P2A - TETon-3G transactivator
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterUbCAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EF1aL-eGFP-W
Plasmid#126686PurposeConstitutive, ubiquitous GFP expression.DepositorInserteGFP
UseLentiviralTagsExpressionMutationPromoterEF1aLAvailable sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-Bxb1-His
Plasmid#123132PurposeBacterial expression plasmid for wild-type Bxb1 with a C-terminal His-tag.DepositorInsertBxb1 integrase
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro
Plasmid#138152PurposeExpresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleusDepositorInserteGFP-MCS
UseLentiviralTags2PEST AND NLSExpressionMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-LDLR-mCherry BlastR
Plasmid#186739PurposeDox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorInsertLDLR (LDLR Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-Calr-KDEL-IRES-Puromycin
Plasmid#134664PurposeExpresses EGFP-tagged endoplasmic reticulum markerDepositorInsertcalreticulin (CALR Human)
UseLentiviralTagsEGFPExpressionMammalianMutationdeleted amino acids 18-413PromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-Hyg-CTCF-N1
Plasmid#131799PurposeExpresses human CTCF-GFP in mammalian cells through transient transfectionDepositorInsertCTCF (CTCF Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF2195
Plasmid#142181PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMLXIPL (MLXIPL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-tagBFP-hTRF2
Plasmid#103803PurposeBLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue lightDepositorUseTagsExpressionMammalianMutationhTRF2: deletion of amino acids 1-42 and 476 compa…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only