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Plasmid#121686PurposepMVP L1-L4 entry plasmid, contains CMV promoter for 3-component MultiSite Gateway Pro assemblyDepositorInsertCMV promoter
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pMEH303 NLS dTomato 6x high affinity MS2 loops
Plasmid#168215PurposeMammalian expression of nuclear-localized dTomato with 6 high affinity MS2 loopsDepositorInsertNLS dTomato
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z33
Plasmid#120050PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with FLAGDepositorInsertFLAG
UsePcr templateTagsFLAGExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::ZeoR-bGHpA
Plasmid#62441PurposeMXS_chaining vector with CMV::ZeoR-bGHpADepositorInsertresistance cassette against Zeocin with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::HygroR-bGHpA
Plasmid#62435PurposeMXS_chaining vector with CMV::HygroR-bGHpADepositorInsertresistance cassette against hygromycin B with CMV Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
CL7-LbCas12a
Plasmid#164658PurposeBacterial expression of LbCas12a using CL7/Im7DepositorInsertLbCas12a
UseTagsThioredoxin, CL7, SUMOExpressionBacterialMutationPromoterT7Available sinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-ÂluxTA2-Â4F
Plasmid#166030PurposeExpresses luxTA(2-Â4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-ÂphlTA2-Â1E
Plasmid#166029PurposeExpresses phlTA(2-Â1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK415-ÂluxTA2-Â4F
Plasmid#165971PurposeExpresses luxTA(2-Â4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, PsynAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE:shRNA A-pGK:rtTA-Cre
Plasmid#68471PurposeInducible lentivirus expressing an arbitrary shRNA and CreDepositorInsertsGFP-shRNA A-miR30
rtTA-2A-Cre
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterpGK and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-Z07
Plasmid#124790PurposeTemplate for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeonGreenDepositorInsertmNeonGreen
UsePcr templateTagsmNeonGreenExpressionMutationPromoterNoneAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_h7SK
Plasmid#102842PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_h7SKpromoter
UseAs pcr templateTagsExpressionMutationPromoterh7SKAvailable sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
MXS_CMV::rtTA3-bGHpA
Plasmid#62445PurposeMXS_chaining vector with CMV::rtTA3-bGHpADepositorInsertcassette containing the reverse tetracycline transactivator with CMV promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEN_TTGmiRc2
Plasmid#25753PurposeEntry vector for cloning miR30-based shRNA driven by TRE-tight promoter with GFP coexpression.DepositorTypeEmpty backboneUseEntry vectorTagsGFPExpressionMutationPromoterAvailable sinceJuly 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
EmptyTranscriptionalReporter
Plasmid#62453PurposeMXS_chaining vector with 2 cassettesDepositorInsertsCMVmin::NLS-Citrine-bGHpA
CMV::NLS-Cherry-bGHpA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB_TRE_AP4-PDGFR_EF1a_eBFP2
Plasmid#186306PurposePlasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.DepositorInsertsAP4-PDGFR
eBFP2
UseTagsHA, mycExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing anti SARS-CoV-2_RBD sybody Sb#14
Plasmid#153522PurposeExpression of the anti SARS-CoV-2_RBD sybody Sb#14DepositorInsertSb#14
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only