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pCMV6-AN-DDK_S497A POLI

Plasmid

#131236

Purpose

Mammalian expression of S497A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S520A POLI

Plasmid

#131237

Purpose

Mammalian expression of S520A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_S580A POLI

Plasmid

#131238

Purpose

Mammalian expression of S580A DNA polymerase iota with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a point mutation converting Pol iota ser…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-DDK_3KA POLI

Plasmid

#131239

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A and K446A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert lysine resi…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-AAVS1-TALEN-L

Plasmid

#159299

Purpose

TALEN (left) targeting the AAVS1 locus with obligate heterodimer FokI

Depositor

Kosuke Yusa

Insert

TALEN targeting the AAVS1

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-AAVS1-TALEN-R

Plasmid

#159300

Purpose

TALEN (right) targeting the AAVS1 locus with obligate heterodimer FokI

Depositor

Kosuke Yusa

Insert

TALEN targeting the AAVS1

Use

TALEN

Tags

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA

Plasmid

#120194

Purpose

Human RPS5 poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-ILVBLpA

Plasmid

#120205

Purpose

Human ILVBL poly(A) site reporter

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-PTEN-3'UTR

Plasmid

#97204

Purpose

Expresses PTEN 3'UTR in mammalian cells

Depositor

Reproducibility Project Cancer Biology

Article

Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

Insert

PTEN 3' UTR (PTEN Human)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m2

Plasmid

#120198

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GFP-RPS5pA-m1

Plasmid

#120196

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pCMVd2-mCherry-Dr-TrkB

Plasmid

#127958

Purpose

Myr mCherryDrTrkB expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al Nat Commun. 2019 Mar 8;10(1):1129. doi:

Insert

Myr-mCherry-3xSAG-Dr-hel-4- TrkB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 1-13 KR

Plasmid

#129299

Purpose

Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed first 13 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 14-22 KR

Plasmid

#129300

Purpose

Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed last 9 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pOEM1_pCMV:deltaN-bmp4-pPH:vsvged

Plasmid

#111158

Purpose

Baculovirus rescue vector, VSVGED pseudotype, hShh

Depositor

Elly Tanaka

Article

Wagner et al Dev Cell. 2017 Mar 27;40(6):608-617.e6.

Insert

deltaNhBmp4 (BMP4 Human)

Use

Baculoviral rescue shuttle vector

Tags

Expression

Insect and Mammalian

Mutation

delta N, mutant BMP lacking the N terminus (delta…

Promoter

CMV

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-M-RAN-H2A2B

Plasmid

#106420

Purpose

M-RAN-H2A2B (RANbody H2A2B::Sm-MYCtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

M-RAN-H2A2B

Use

Tags

HA, His, and Multiple MYC

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-H2A2B

Plasmid

#106421

Purpose

Y-RAN-H2A2B (RANbody H2A2B::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-H2A2B

Use

Tags

HA and His

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M1 N184X

Plasmid

#92365

Purpose

expression of M1 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

1-194 bp of 5'UTR deleted, N184X

Promoter

CMV

Available since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN51 (L1L2)

Plasmid

#80108

Purpose

Late1(delta)-O3-33-myc-PH

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (L1L2)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
pCMV-EPN51 (R40A)

Plasmid

#80109

Purpose

Late1-O3-33-myc-PH(R40A)

Depositor

Neil King

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-51 (R40A)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only