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Plasmid#201885PurposeUsed for bacterial expression of human protein ARF4DepositorInsertARF4 (ARF4 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET3-ARF5-His6(CO)
Plasmid#201886PurposeUsed for bacterial expression of human protein ARF5DepositorInsertARF5 (ARF5 Human)
UseTags6xHis (GSGSGSHHHHHH)ExpressionBacterialMutationCodon optimized for bacterial expressionPromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 omega1 Tnos:nptII:Pnos - SF - P35S:hCas9:Tnos (GB1657)
Plasmid#160640PurposeModule for the expression of the human codon optimized Cas9 and kanamycin resistance (NptII).DepositorInsertTnos:NptII:Pnos-SF-P35s:hCas9:tNos
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterPnos, 35SAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 HLA-DQA2 WW domain
Plasmid#104297PurposeBacterial expression of WW domain from HLA-DQA2DepositorInsertHLA-DQA2 WW domain (HLA-DQA2 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPS13D(∆ATG2-C/PH)^EGFP
Plasmid#194004PurposeDeletion of DHPH domain of VPS13DDepositorInsertCodon optimized VPS13D (VPS13D Human)
UseTagsEGFPExpressionMammalianMutationearly stop codon before DHPHPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-ABERA-P2A-Puro
Plasmid#112675PurposeLentivirus for constitutive expression of ABE7.10 RA (codon optimized)DepositorInsertABERA
UseLentiviralTagsFLAGExpressionMutationD10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Cas9-P2A-Puro
Plasmid#110837PurposeLentiviral vector for expression of Cas9 in mammalian cells (codon optimized)DepositorInsertCas9
UseLentiviralTagsExpressionMutationWTPromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3RA-PGK-Puro
Plasmid#110846PurposeLentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE4GamRA-P2A-Puro
Plasmid#112673PurposeLentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)DepositorInsertBE4GamRA
UseLentiviralTagsFLAGExpressionMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
(564) pAAV Alb-AAT KRAB-SadCas9 U6-mPcsk9
Plasmid#163025PurposeExpression of CRISPRi with gRNA against mouse Pcsk9DepositorInsertMammalian codon-optimized SadCas9 (Pcsk9 S. aureus)
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE3-FNLS(RA)
Plasmid#112671PurposeCMV expression vector for BE3-FNLS construct (codon optimized)DepositorInsertBE3-FNLS
UseTags3x FLAGExpressionMammalianMutationNLS sequence at the N-terminus and D10APromoterCMVAvailable sinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE4RA-P2A-Puro
Plasmid#112672PurposeLentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)DepositorInsertBE4RA
UseLentiviralTagsFLAGExpressionMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-BE3-PGK-Puro
Plasmid#110845PurposeLentiviral vector for dox-inducible expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterTRE3GAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-Puro
Plasmid#110839PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-PGK-Puro
Plasmid#110843PurposeLentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-SpCas9-HF1 (without sgRNA)
Plasmid#92102PurposeExpression plasmid for human codon-optimized high-fidelity SpCas9-HF1, px330-like backbone (without U6-sgRNA coding sequence)DepositorInsert3xFlag-NLS-Streptococcus pyogenes Cas9-HF1-NLS
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN497A, R661A, Q695A, Q926APromoterCbhAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-GFP-PGK-Puro
Plasmid#110867PurposeLentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-HF1-PGK-Puro
Plasmid#110857PurposeLentiviral vector for constitutive expression of Cas9-HF1 (not codon optimized)DepositorInsertCas9-HF1
UseLentiviralTags3X FLAGExpressionMutationN497A, R661A, Q695A, Q926APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-PGK-Puro
Plasmid#110842PurposeLentiviral vector for constitutive expression of BE3 (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only