-
Plasmid#207680PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pGL4-23-NegCtrl
Plasmid#163904PurposeNegative control vector for luciferase reporter assaysDepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterminimalAvailable sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR
Plasmid#187089PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-SF-iGluSnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_PEBP1-Halo
Plasmid#175333PurposeProduction of HaloTag fusion proteinDepositorInsertPEBP1 (PEBP1 Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TagBFP-P2A-Pre-SPHERE-iGABASnFR
Plasmid#187091PurposeGABA sensor for localization to membrane contatctDepositorArticleInsertPre-SPHERE-iGABASnFR
UseTagsTagBFP-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iRFP-P2A-Post-SPHERE-iGABASnFR
Plasmid#187092PurposeGABA sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-iGABASnFR
UseTagsiRFP670-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-TEV-pro-conA
Plasmid#159527PurposeExpresses pro-concanavalin A, a lectin from Canavalia ensiformis, in E. coli. Includes N-terminal TEV recognition site. Native N-terminal sequence is reconstituted upon TEV cleavage.DepositorArticleInsertCanavalia ensiformis pro-concanavalin A
UseTagsSix-Histidine tag and tobacco etch virus (TEV) pr…ExpressionBacterialMutationPromoterT5Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-iRFP-P2A-Post-SPHERE-SF-iGluSnFR
Plasmid#187090PurposeGlutamate sensor for localization to membrane contatctDepositorArticleInsertPost-SPHERE-SF-iGluSnFR
UseTagsiRFP670-P2AExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-Cas3
Plasmid#209427PurposePlasmid for CASCADE-Cas3 based genome engineering of streptomycetesDepositorInsertcodon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterPtipA; crRNA under control of PermE*Available sinceMarch 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Pink Flamindo-p2a-paGFP-bPAC-CAAX
Plasmid#202718PurposeMammalian co-expression of soluble Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imagingDepositorArticleInsertsPink Flamindo
paGFP-bPAC-CAAX
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
UseTagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJAT56
Plasmid#204300PurposeFExpress phiC31 integrase under heat shock control. Insert marked with 3XP3-EGFP, which expresses in eyes.DepositorInsert3XP3-EGFP
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-dSa-VP64
Plasmid#99680PurposeExpresses dSa Cas9 fused to gold standard VP64 activatorDepositorArticleInsertdCas9
UseAAVTagsVP64ExpressionMutationdead Cas9PromoterAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL0_16 [2μ ORI]
Plasmid#198929PurposeLevel 0 partDepositorInsert2μ ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDN3p
Plasmid#162457PurposePositive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.DepositorInsertAga2p-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsALFA tag, HA and myc tags, and eUnaG2 fluorescent…ExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
JGE302_pAAV-hSyn-DIO-Myc-PYLcs-bArrestin2-YFP (p2)
Plasmid#107247Purposebeta arrestin 2 fused to PYLcs for abscisic acid mediated recruitment of ABIcs labelled proteins.DepositorArticleInsertARRB2
UseAAVTagsMYC, PYLcs, and YFPExpressionMutationPromoterhSYNAvailable sinceApril 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_13 [pIP501 ORI]
Plasmid#198927PurposeLevel 0 partDepositorInsertpIP501 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-DAT-myc
Plasmid#107457PurposeMammalian expression plasmid for codon optimized hDAT with internal MYC tag inserted in ECL2DepositorInsertDopamine Transporter (DAT) (SLC6A3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Zeo
Plasmid#207732PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a moxGFP-2A-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits