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CAG-Otx2-IRES-GFPd2

Plasmid

#73996

Purpose

Plasmid expressing Otx2(isoform b)-IRES-GFPd2

Depositor

Connie Cepko

Article

Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

Insert

Otx2(isoform b)-Ires-GFP (Otx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS/XBB.1.5-RBD

Plasmid

#213038

Purpose

Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.XBB.1.5 variant

Depositor

Priyamvada Acharya

Article

Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

Insert

pCAGGS/XBB.1.5-RBD (S )

Use

Tags

6 His Tag

Expression

Mammalian

Mutation

G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

Promoter

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-Bi-DREADD

Plasmid

#159457

Purpose

AAVS1 targeting donor plasmid with Bi-DREADD expression cassette and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

Bi-DREADD

Use

Tags

Expression

Mammalian

Mutation

fusion protein of hM3Dq-mCherry and HA-KORD seper…

Promoter

CAG

Available since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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AAV pCAG-mRuby3-WPRE

Plasmid

#107744

Purpose

Can be used to express mRuby3. Can also be used to create adeno-associated virus for delivery of the mRuby3 sequence.

Depositor

Rylan Larsen

Article

Zhuang et al Cell Rep. 2021 Oct 12;37(2):109826. doi:

Insert

mRuby3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-Sox2-IRES-Neo

Plasmid

#13462

Depositor

Shinya Yamanaka

Article

Tokuzawa et al Mol Cell Biol. 2003 Apr . 23(8):2699-708

Insert

Sox2 (Sox2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG-loxpSTOPloxp-lyn-NeonGreen

Plasmid

#175257

Purpose

membrane target protein labelling

Depositor

Frank Bradke, Sebastián Dupraz

Article

Alfadil et al J Vis Exp. 2021 Oct 14;(176). doi: 10.37

Insert

Lyn membrane targeting motif

Use

Cre/Lox

Tags

mNeonGreen

Expression

Mammalian

Mutation

None (wt)

Promoter

pCAG

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Gag-FKBP-Myc

Plasmid

#177909

Purpose

HIV Gag-FKBP-Myc tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

HIV Gag (gag )

Use

Tags

FKBP12 and Myc tag

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-FLEX-rc[Chronos-GFP]

Plasmid

#59056

Purpose

Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-nDsRedFL-miR9/9*/124

Plasmid

#177807

Purpose

The pre-miR-9/9* and pre-miR-124 sequences have been inserted into the EcoRV of pCAG-nDsRedFL-intron.

Depositor

Rikako Sanuki

Article

Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

Insert

miR-9/9*/124

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-F-bpA-2xSV40pA-F-td-sfGFP

Plasmid

#133572

Purpose

CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 2x SV40pA stop)

Depositor

Mario Capecchi

Insert

Tandem dimer superfolder GFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SARS2-S-D614 (NC-flag)

Plasmid

#156418

Purpose

Mammalian expression of SARS-CoV-2 S protein (D614) with the flag tag at N- and C-termini. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.

Depositor

Michael Farzan, Hyeryun Choe

Article

Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

Insert

SARS-CoV-2 S protein (S SARS CoV2)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Use

Tags

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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YA1531: pAAV_CAG-FLEX-QuasAr3

Plasmid

#107701

Purpose

in vivo voltage imaging

Depositor

Adam Cohen

Article

Adam et al Nature. 2019 May 1. pii: 10.1038/s41586-

Insert

QuasAr3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-MLC2(CA)-IRES-GFP

Plasmid

#133928

Purpose

Bicistronic vector for the expression of EGFP reporter and untagged constitutive active myosin II light chain

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

EGFP and MLC2(CA) (MYL2 Human)

Use

Tags

Expression

Mammalian

Mutation

MLC2 T18D, S19D

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-GBP6-10gly-VPminx4

Plasmid

#50796

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-VPminx4 activation domain fusion protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-rat Nrxn1beta AS4(-)

Plasmid

#58267

Purpose

Expression plasmid for HA-tagged rat Neurexin 1beta, lacking the insert at alternatively spliced segment 4 (AS4-)

Depositor

Peter Scheiffele

Article

Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

Insert

Nrxn1beta AS4(-) (Nrxn1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

see comments

Promoter

CAG

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-RiboL1-jGCaMP8m

Plasmid

#192613

Purpose

AAV transfer plasmid for CAG mediated expression of soma-targeted (RiboL1) jGCaMP8m.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8m

Use

AAV

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-CAG-3xNLS-AausFP1

Plasmid

#191096

Purpose

To express a bright green FP to label eucaryotic cell nuclei. To be used in tissue-clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-AausFP1

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

Optimized to Human codon usage

Promoter

CMV enhancer and CAG

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PB CAG-F-bpA-3xSV40pA-F-td-sfGFP

Plasmid

#133573

Purpose

CAG tdGFP FLP/FRT reporter piggyBac transposon (bpA 3x SV40pA stop)

Depositor

Mario Capecchi

Insert

Tandem dimer superfolder GFP

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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