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pDONR221-ARL11-no stop

Plasmid

#67341

Purpose

hArl11 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

bp 442 T to C; aa C148R

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEMS2026

Plasmid

#49133

Purpose

AAV plasmid with TNNT1-B (Ple301) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple301-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TNNT1-B

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pSUPER-retro-puro-Smad3

Plasmid

#89828

Purpose

Knockdown human Smad3 expression in human mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

Insert

SMAD3 shRNA (SMAD3 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEMS1988

Plasmid

#49119

Purpose

AAV plasmid with CAGGS promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mPapaya1-Calnexin-N-14

Plasmid

#56350

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Calnexin (CANX Human)

Use

Tags

mPapaya1

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL11

Plasmid

#67340

Purpose

hArl11 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GST-MED9

Plasmid

#15408

Depositor

Ronald Conaway, Joan Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED9 (Med9 Mouse)

Use

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Promoter

Available since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-Med9 pBacPAK8

Plasmid

#15363

Depositor

Ronald Conaway, Joan Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

Med9 (Med9 Mouse)

Use

Baculovirus construction

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCDSRaD-sdi1

Plasmid

#219566

Purpose

Mammalian expression of human sdi1 (p21 CDK inhibitor)

Depositor

James R Smith, Asao Noda

Article

Noda et al Exp Cell Res. 1994 Mar;211(1):90-8. doi:

Insert

sdi1 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SRa

Available since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Luciferase-CD4-bla

Plasmid

#189981

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hPER1-Halo-CD4-bla

Plasmid

#189982

Purpose

Donor Vector to integrate HaloTag C-terminal into the human PER1 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-mScarlet-I-CD4-bla

Plasmid

#189984

Purpose

Donor Vector to integrate mScarlet-I C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

mScarlet-I

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Luciferase-CD4-bla

Plasmid

#189985

Purpose

Donor Vector to integrate firefly Luciferase C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Luciferase

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pDB2-hCRY2-Halo-CD4-bla

Plasmid

#189986

Purpose

Donor Vector to integrate HaloTag C-terminal into the human CRY2 Locus to express it as a fusion protein

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

HaloTag

Use

CRISPR

Tags

3xFlag

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiCriprV2-sgRNA-PER1-#2

Plasmid

#189988

Purpose

Lentiviral Cas9/sgRNA vector targeting C-terminus of hPER1, works with Addgene 189979-189982

Depositor

Achim Kramer

Article

Gabriel et al Proc Natl Acad Sci U S A. 2024 Aug 20;12

Insert

Period1 (PER1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pMtnA FLAG-IntS7 puro

Plasmid

#208405

Purpose

Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

pMT FLAG MCS puro (defl Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

MtnA

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Gαi3-91-NLuc

Plasmid

#207150

Purpose

Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

Depositor

Tom Van Loy, Dominique Schols

Article

Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

Insert

GNAI3 (GNAI3 Human)

Use

Tags

Expression

Mammalian

Mutation

NLuc inserted at position L(91)K

Promoter

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3319

Plasmid

#144795

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZSCAN5A (ZSCAN5A Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1278

Plasmid

#144103

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF587 (ZNF587 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF1277

Plasmid

#144102

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF587 (ZNF587 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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