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pICXC3H

Plasmid

#49022

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pCAndy-CBioTAP

Plasmid

#53570

Purpose

Plasmid vector carrying C-Bio-1xproteinA tag

Depositor

Mitzi Kuroda

Article

Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Aug. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXNH3

Plasmid

#49024

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJT103

Plasmid

#25087

Depositor

Christopher Voigt

Article

Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

Insert

PompC-lacZ

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2010

Availability

Academic Institutions and Nonprofits only

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mEos-A69V-Actin-7

Plasmid

#57334

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Actin (ACTB Human)

Use

Tags

mEos A69V

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pISH-Pknox2

Plasmid

#74354

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Pknox2. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Pbx/knotted 1 homeobox 2 (Pknox2 )

Use

Tags

Expression

Mutation

Promoter

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only

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pISH-Trpm5

Plasmid

#105993

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Trpm5 (Trpm5 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYEXC3H

Plasmid

#49026

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pYEXNHG3

Plasmid

#49025

Purpose

FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pZS1(pLtetO-TP901-aav-proA-tetR)-katGp-PhiC31-proD-oxyR

Plasmid

#78228

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Article

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic gene circuit

Use

Tags

Expression

Mutation

Promoter

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only
pCSDC2-donor

Plasmid

#113813

Purpose

CRISPR donor plasmid to tag human transcription factor CSDC2 with GFP

Depositor

Kevin White

Insert

CSDC2 homology arms flanking EGFP-IRES-Neo cassette (CSDC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pISH-Plcb2

Plasmid

#105991

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

Plcb2 (Plcb2 Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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mPA-GFP-FYN-N-10

Plasmid

#57135

Purpose

Localization: Membrane Associated Kinase, Excitation: 400 / 504, Emission: 515 / 517

Depositor

Michael Davidson

Insert

FYN (FYN Human)

Use

Tags

mPA-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pWUKI Cas1 P279L Cas2

Plasmid

#80095

Purpose

Encodes Cas1 P279L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Use

Tags

Expression

Bacterial

Mutation

P279L

Promoter

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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FXN_Halo_N_allele

Plasmid

#178141

Purpose

Donor vector for endogenous tagging of human FXN at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (FXN Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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COQ2_Halo_N_allele

Plasmid

#178154

Purpose

Donor vector for endogenous tagging of human COQ2 at the N-terminus with halotag

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Halotag (COQ2 Human)

Use

Donor vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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PsodA-cfp

Plasmid

#127945

Purpose

CFP fluorescent reporter for SodA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PsodA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PacrAB-cfp

Plasmid

#127944

Purpose

CFP fluorescent reporter for AcrAB

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PacrAB-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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P_70-cfp

Plasmid

#127942

Purpose

CFP fluorescent reporter for _70

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

P_70-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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PinaA-cfp

Plasmid

#127937

Purpose

CFP fluorescent reporter for InaA

Depositor

Mary Dunlop

Article

Rossi et al Commun Biol. 2019 Jul 11;2:259. doi: 10.

Insert

PinaA-cfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge