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Plasmid#187155Purposemammalian cell expression of HUWE1-3xFLAGDepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody
Plasmid#49172PurposeBacterial expression of GFP nano bodyDepositorInsertGFP nanobody
UseTagsHHHHHHExpressionBacterial and MammalianMutationPromoterT7Available sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP/CALR wt-FLAG
Plasmid#204525PurposeRetroviral expression of human CALR wt-FLAGDepositorInsertCALR wt-FLAG (CALR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag
Plasmid#209666PurposeNon-responsive controlDepositorInsertcpSFGFP.HaloTag
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFVdelNS1-Cre
Plasmid#179952PurposepCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with CreDepositorInsertYFV-17D lacking nonstructural protein 1 (NS1), but with Cre
UseTransneuronal tracingTagsExpressionMutationPromoterSP6Available sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1211YN
Plasmid#61704PurposeGateway compatible BiFC vector (pEarleyGate201-YN)DepositorTypeEmpty backboneUseBifc assayTagsYFP-N and HAExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGTQL1221YC
Plasmid#61705PurposeGateway compatible BiFC vector (pEarleyGate202-YC)DepositorTypeEmpty backboneUseBifc assayTagsYFP-C and FLAGExpressionPlantMutationPromoter35SAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 HA-YY1
Plasmid#104395PurposeHA-YY1DepositorInsertYY1 (YY1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV p16 Neo (w111-1)
Plasmid#22260DepositorInsertcyclin-dependent kinase inhibitor 2A (CDKN2A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3,5)P2 Probe
Plasmid#211512PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3,5-Bisphosphate (PI(3,5)P2) from bacteriaDepositorInsertSnxA-2xPX
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-EGFP-Tau P301L
Plasmid#46908Purposeexpresses EGFP tagged Tau P301L in mammalian cellsDepositorInsertTau P301L (MAPT Human)
UseTagsEGFPExpressionMammalianMutationP301LPromoterCMVAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
Anti-GFP [N86/38.1R]
Plasmid#114492PurposeMammalian Expression Plasmid of anti-GFP. Derived from hybridoma N86/38.1.DepositorInsertanti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
UseTagsExpressionMammalianMutationPromoterdual CMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDA_052
Plasmid#136474PurposeLentiviral expression of AsCas12a gRNADepositorInsertPuromycin resistance
UseLentiviral and Synthetic BiologyTagsExpressionMutationPromoterEF1aAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUGW-PercevalHR
Plasmid#49083PurposeLentiviral vector for PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.DepositorInsertPercevalHR
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
GST-GGA3-VHS-GAT
Plasmid#173719PurposeVHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.DepositorUseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT-EF-Pdgfralpha-EGFPN
Plasmid#66787PurposeExpression of murine PDGFRalpha tagged with GFP under the control of EF1a promoterDepositorInsertPDGFRalpha (Pdgfra Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGPA1
Plasmid#115476PurposeGeneration of mariner-based transposon mutant libraries in Enterococcus faeciumDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestGFP_HuD
Plasmid#65763Purposeexpresses GFP-tagged HuD in mammalian cellsDepositorInsertELAVL4 (ELAVL4 Human)
UseTagsFLAG HA and V5ExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC18R6KT-mini-Tn7T-Km
Plasmid#64969Purposemini-Tn7 base vector with transcriptional terminator and oriTDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
lentiCRISPRi(v2)-Blast
Plasmid#170068PurposeExpresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistanceDepositorInsertKRAB-dCas9-MeCP2
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET21b-P35
Plasmid#11808DepositorInsertP35
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-mCherry
Plasmid#114472PurposemCherry ControlDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertmCherry
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-R-gfp
Plasmid#220927PurposeExpresses gfp using J23119 promoter and pgRNA_AT backboneDepositorInsertsuperfolder GFP
UseTagsFLAGExpressionBacterialMutationPromoterJ23119Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_ARRB2-1-383-CTEV-2xHA
Plasmid#194382PurposeSplit TEV assaysDepositorInsertARRB2-CTEV-2xHA (ARRB2 Human, Synthetic)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-SEP-GluA1 TKIT
Plasmid#169441PurposeExpression of 2 guides + donor DNADepositorInsertSuper ecliptic pHluorin (SEP)
UseAAV and CRISPRTagsExpressionMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pECIA14
Plasmid#47051PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tagsDepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsAlkaline Phosphatase (human, placental), Flag, HR…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
Plasmid#209723PurposeExpresses mitochondrially targeted low affinity ratiometric ATP sensorDepositorInsert(mito).iATPSnFR2.A95A.A119L.miRFP670nano3
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_502
Plasmid#96923Purposefor CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSFDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.LifeAct-Ruby.W
Plasmid#51009PurposeLentivector that expresses LifeAct-Ruby from internal human PGK promoterDepositorInsertLifeAct-mRFPruby
UseLentiviralTagsLifeActExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pmiRFP680-N1
Plasmid#136557PurposeA cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)DepositorInsertmiRFP680
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Apple-53BP1trunc
Plasmid#69531PurposeFluorescent reporter for DNA damageDepositorInsert53BP1 (TP53BP1 Human)
UseLentiviralTagsApple fluorescent proteinExpressionMammalianMutationPromoterCMVAvailable sinceOct. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXF89
Plasmid#139030PurposeMosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette systemDepositorInserthalotag (no STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_MANF-Halo
Plasmid#175341PurposeProduction of HaloTag fusion proteinDepositorInsertMANF (MANF Human)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)
Plasmid#103870PurposeHigh specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.DepositorInsertsUseCRISPRTagsGSGGGGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLXSN16E6E7
Plasmid#52394PurposeThe plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.DepositorInsertHPV16 E6E7
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
EF.CMV.RFP
Plasmid#17619DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only