159,557 results

1. Cre-IRES-PuroR

   Plasmid

   #30205

   Purpose

   Lentiviral coexpression of Cre and puromycin from the human EF1a promoter

   Depositor

   Darrell Kotton

   Article

   Somers et al Stem Cells. 2010 Oct . 28(10):1728-40.

   Insert

   Cre recombinase (cre Enterobacteria phage P1)
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   April 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ePPEplus

   Plasmid

   #205241

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Detailed in manuscript

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF_PE2

   Plasmid

   #199267

   Purpose

   Mammalian expression of SpCas9 prime editor 2 under EF1α promoter

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   PE2-P2A-mp53DD
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Δ40-903

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HS69 pComb3xss anti-Homer1 nanobody

   Plasmid

   #134716

   Purpose

   Bacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabel

   Depositor

   James Trimmer

   Article

   Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

   Insert

   anti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
   

   Use

   Tags

   6xHis-HA

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEGFP-parkin WT

   Plasmid

   #45875

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET21a-Streptavidin-Alive

   Plasmid

   #20860

   Purpose

   Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

   Depositor

   Alice Ting

   Article

   Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

   Insert

   Streptavidin-Alive
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Wt gene

   Promoter

   Available since

   April 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC57-CmYLCV

   Plasmid

   #205245

   Purpose

   For plant prime editing in wheat plants or monocotyledons protoplasts

   Depositor

   Zong Yuan

   Article

   Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

   Insert

   CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCV

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV.rTH.PI.Cre.SV40

   Plasmid

   #107788

   Purpose

   AAV vector expressing Cre from rTH promoter

   Depositor

   James M. Wilson

   Has Service

   AAV Retrograde and AAV9

   Insert

   Cre
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   rTH

   Available since

   May 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TFORF1939

   Plasmid

   #141893

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MEIS1 (MEIS1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. P362-FBP_12

    Plasmid

    #139703

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    various

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-2E2-HA-mEGFP

    Plasmid

    #129595

    Purpose

    The encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pJAM1.19_PB-CIT-PGK-Blast

    Plasmid

    #205564

    Purpose

    Express human CIT

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    CIT (CIT Human)
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-ABCB1_STOP

    Plasmid

    #221424

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Wolf et al Cell Chem Biol. 2024 Dec 24:S2451-9456(2

    Insert

    ABCB1 (ABCB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLentiPGK Blast DEST JNKKTRmRuby2

    Plasmid

    #59154

    Purpose

    Lentiviral vector to express JNK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human)
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pNeonGreen-C1-Nir1-LNS2

    Plasmid

    #220083

    Purpose

    high affinity PA biosensor with green fluorophore

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    NeonGreen-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-(-2)AP-Ub

    Plasmid

    #232582

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEHAC1-HA-BirAonly

    Plasmid

    #232584

    Purpose

    Express HA-BirA

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    BirA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5-AP(-3)-Ub

    Plasmid

    #232583

    Purpose

    Express Ubiquitin tagged with a modified Acceptor Peptide (AP)

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    Ubiquitin
    

    Use

    Tags

    AP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEHAC1-CHIP-GSGS-BirA-HA

    Plasmid

    #232588

    Purpose

    Express BirA tagged to the C-terminus of CHIP with a GSGS linker between BirA and CHIP.

    Depositor

    Sagar Bhogaraju

    Article

    Mukhopadhyay et al Sci Adv. 2024 Aug 9;10(32):eadp3000. doi

    Insert

    CHIP - BirA (CHIP Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge