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Showing: 1301 - 1320 of 8972 results

  9. pML104

    Plasmid
    #67638
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains URA3 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67638-plasmid-map-sequence-id-322882

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  10. pML107

    Plasmid
    #67639
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformation
    Depositor
    John Wyrick
    Article
    Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1
    Inserts
    Cas9
    single guide RNA expression cassette
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pSNR52 and pTDH3 (aka GAP promoter)
    Available since
    Aug. 31, 2015
    Availability
    Academic Institutions and Nonprofits only
    67639-plasmid-map-sequence-id-220324

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  11. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail

    Plasmid
    #60392
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoter
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Budding Yeast, Synthetic)
    Use
    Tags
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4), amino a…
    Promoter
    GAL
    Available since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pUDP123

    Plasmid
    #107269
    Purpose
    pUDP123 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes OpADE2 and OpNIAD and Spcas9D147Y P411T in O. parapolymorpha (HH-gRNAOpADE2-HDV-linker-HH-gRNAOpNIAD-HDV)
    Depositor
    Jean-Marc Daran
    Article
    Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4
    Insert
    polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting OpADE2 and NIAD in O. parapolymorpha
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    ScTDH3
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    107269-plasmid-map-sequence-id-208617

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  17. GFP-ATG8(416)/GFP-AUT7(416)

    Plasmid
    #160389
    Purpose
    Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selection
    Depositor
    John McCusker
    Insert
    autophagy-related 8 (ATG8 Budding Yeast)
    Use
    Cen/ars
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Endogenous ATG8 promoter
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160389-plasmid-map-sequence-id-314524

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  20. pBCSK_LEU2_I

    Plasmid
    #109060
    Purpose
    yeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposes
    Depositor
    Colin Logie
    Insert
    LEU2 (LEU2 Zygosaccharomyces rouxii, Budding Yeast)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    LEU2
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109060-plasmid-map-sequence-id-209546

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Showing: 1301 - 1320 of 8972 results