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Plasmid#82502PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. G418 resistance is encoded by the Meo gene.DepositorInserteGFP-IRESMeo
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-MCU-Flag-TetOFF
Plasmid#185657PurposeTet/dox-repressible expression of C-terminus Flag tagged human mitochondrial calcium uniporterDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
8024 GFP_Kras_G12C_2A_puro
Plasmid#64373PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2A oncogene along with puro resistance geneDepositorInsertmouse Kras2A (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8027_GFP-KrasG12C_2B_puro
Plasmid#64372PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
8047_GFP-Kras2B_ires_puro
Plasmid#64371PurposeThis a retroviral expression plasmid expressing GFP tagged wild type Kras 2B oncogene along with puro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationnonePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCU-mCerulean
Plasmid#185809PurposeHuman mitochondrial calcium uniporter with C-terminus mCerulean tagDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
8798 GFP-KrasG12C_2B_hygro
Plasmid#64376PurposeThis a retroviral expression plasmid expressing GFP tagged G12C mutant Kras 2B oncogene along with hygro resistance geneDepositorInsertmouse Kras2B (Kras Mouse)
UseRetroviralTagsGFPExpressionMutationchanged glycine 12 to cysteinePromoterAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA0482
Plasmid#96968Purposeexpression of methicillin-resistant Staphylococcus aureus orf 0482DepositorInsertMRSA ORF0482
UseTagsN-ter TEV protease cleavable 6HisExpressionBacterialMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL11017 (pICH47732::NOSp-BAR-NOST)
Plasmid#51145PurposeConfers Basta resistance in plantaDepositorInsertNOSp-BAR-NOST
UseTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A_mmFbxw7(res) (closed)
Plasmid#160105PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorInsertFbxw7alpha (Fbxw7 Mouse)
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pCW57.1-FRB-MCU-Flag-TetOFF
Plasmid#185659PurposeTet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitopeDepositorInsertMitochondrial calcium uniporter (MCU Human)
UseLentiviralTagsFRB and FlagExpressionMammalianMutationPromoterpTight TREAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX304_zeo_mmFbxw7(res)
Plasmid#160106PurposeExpresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNADepositorInsertFbxw7alpha (Fbxw7 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_COF2F04
Plasmid#70901PurposeGateway entry cloneDepositorInsertmod(mdg4) (mod(mdg4) Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCambia-loxKan
Plasmid#199590Purposebasic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibilityDepositorInsertFRT-lox66-NosP-KanR-NosT-lox71-FRT
UseCre/LoxTagsExpressionPlantMutationPromoterNosAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET21b-C-MBP
Plasmid#214304PurposeProvide a bacterial expression vector that encodes C-terminus MBP tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJK295
Plasmid#71561PurposeProduction of Acetobacter aceti 1023 thioredoxin (AaTrx)DepositorInsertthioredoxin
UseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYGA
Plasmid#217672PurposeFor expression of SUMO tagged human gamma A crystallinDepositorInsertCRYGA (CRYGA Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETB BS2mT4L1
Plasmid#141203PurposeChimeric protein of inactive T4L lysozyme and an estarase from Bacillus subtilis.TEV cleaving site after the N-terminal Histag.DepositorInsertBS2m-T4L1
UseTagsExpressionBacterialMutationE11QPromoterAvailable sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GW
Plasmid#112902PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only