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Plasmid#180335Purposemammalian expression of human SEPT9_i1 DN7 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 DN7PromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 Gmut-msfGFP
Plasmid#180331Purposemammalian expression of human SEPT9_i1 Gmut fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 W520A, H530DPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 NCmut2-msfGFP
Plasmid#180330Purposemammalian expression of human SEPT9_i1 NCmut2 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 I281D, M288DPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AiP906-pCMV-oBxb1
Plasmid#181777PurposeBxb1-in vectorDepositorInsertoBxb1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_PM
Plasmid#177751PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iDianiSnFR_ER
Plasmid#177750PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iDianiSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_PM
Plasmid#177747PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_PM
Plasmid#177745PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_PM
Plasmid#177743PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_PM
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCyt_BrEt_SnFR_ER
Plasmid#177746PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCyt_BrEt_SnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
_pCMV(MinDis)-iCyt_F_SnFR_ER
Plasmid#177744PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsert_pCMV(MinDis)-iCyt_F_SnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis)-iCytSnFR_ER
Plasmid#177742PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonistsDepositorInsertpCMV(MinDis)-iCytSnFR_ER
UseTagsExpressionMammalianMutationPromoterhCMVAvailable sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-PGRMC1-21-195
Plasmid#173473PurposePGRMC1 lacking the N-terminal region upstream of the transmembrane regionDepositorInsertPGRMC1 (PGRMC1 Human)
UseTagsHAExpressionMammalianMutationDeleted for amino acids 1-20PromoterAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-luc-miR-1
Plasmid#171556Purposea positive control reporter with 3' UTR sequences perfectly complementary to miR-1DepositorInserta sequences perfectly complementary to miR-1
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-null (BPK1077)
Plasmid#171128PurposePlasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.DepositorInsertpCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterpCMV and T7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-NG-nCas9
Plasmid#163539PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-NG-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31ANG (L111R, D1135V, G1218R, E…PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_eA3A T31A-nCas9
Plasmid#163538PurposeMammalian CG-to-GC base editingDepositorInserteA3A T31A-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)eA3A T31APromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-EE-UdgX-nCas9
Plasmid#163564PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-EE-UdgX-nCas9
UseCRISPRTagsExpressionMammalianMutationnCas9 (D10A)EE (R126E, R132E)PromoterAvailable sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_UBM1*2* POLI
Plasmid#131232PurposeMammalian expression of an N-terminally FLAG-tagged DNA polymerase iota mutant, containing point mutations in both UBM domainsDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains inactivating mutations in both ubiquitin…PromoterCMV enhancer + CMV promoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-DDK_S433A POLI
Plasmid#131235PurposeMammalian expression of S433A DNA polymerase iota with an N-terminal FLAG-tagDepositorInsertDNA polymerase iota (POLI Human)
UseTagsFLAGExpressionMammalianMutationContains a point mutation converting Pol iota ser…PromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only