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Plasmid#118470PurposecpBFP-based single-color biosensor for monitoring Protein Kinase A activity.DepositorInsertblueAKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI
Plasmid#115207PurposeExpresses BzoCas13b from a SUMO-tagged bacterial expression constructDepositorInsertBzoCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
6xAP-1_RE::GrRenilla
Plasmid#124534PurposeAP-1 GreenRenilla luciferase reporterDepositorInsertAP-1GreenRenilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-ICUE
Plasmid#118480PurposeddRFP-based single-color biosensor for monitoring cAMP dynamics.DepositorInsertRAB-ICUE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-PKI-GFP
Plasmid#118482PurposeGFP-tagged Protein Kinase A inhibitor peptide.DepositorInsertPKI-GFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; ITagsExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141A2.0
Plasmid#99896PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU6Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
EKARdual
Plasmid#99870PurposeMeasure ERK activity by conformational change after ERK substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertEKAR
UseTagsShadowG and mTFP1ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-3'UTR-Tetra-com-vector
Plasmid#132553PurposeExpresses spCas9 and sgRNA scaffold with com aptamer replacing the TetraloopDepositorInsertsCas9
sgRNA scaffold with com replacing the Tetraloop
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)
Plasmid#192124PurposepHR-OFF VIPER CAR (anti-CD19)-candidate design(d+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ154
Plasmid#69305PurposeGateway entry vector with AteCas9 (Arabidopsis codon optimized)DepositorInsertAteCas9 (Arabidopsis codon optimized)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-M-Gal4
Plasmid#84554PurposeMESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivatorDepositorInsertRap-MESA-FRB-20-M-Gal4
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D2.0
Plasmid#99906PurposeExpress single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterOsU3Available sinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-OFF VIPER CAR (anti-CD19)-candidate (c+iii)
Plasmid#192122PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(c+iii)DepositorInsertpEF1a-anti-CD19scFV_CD28_peptide_P2A_DAP10_NS3(s139a)_CD3z-mCherry
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro
Plasmid#192683PurposeLentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNAs #1,2,3 (MYOD1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
TB:7xE-Box:MiniP
Plasmid#133880Purpose7 copies of the E-box motifDepositorInsert7 copies of the E-box motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode1
Plasmid#83678Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode1
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-5xUAS-sfGFP-GMRWhite
Plasmid#165907PurposeBlasticidin resistant 5xUAS sfGFP GMR-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screeningDepositorInsertSelectable UAS Response Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only