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Plasmid#207703PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-miniTurbo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits
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qTAG-C-V5-Puro
Plasmid#207730PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Zeo-3xFLAG
Plasmid#207706PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Blast-3xHA
Plasmid#207685PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-3xHA cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
3xmScarletI_UtrCH
Plasmid#112962PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertUtrCH
UseTags3x mScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-moxGFP
Plasmid#207688PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-moxGFP cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pENTR1a-CRISPRoffv2.1
Plasmid#207174PurposeGateway Cloning Vector for CRISPRoffv2.1DepositorInsertCRISPRoffv2.1
UseCRISPRTagsTagBFPExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EgI-NeoR
Plasmid#207175PurposeGateway compatible Lentiviral Expression Plasmid EF1a-IRES-NeoRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EgI-HygroR
Plasmid#207177PurposeGateway compatible Lentiviral Expression Plasmid EF1a-IRES-HygroRDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3 Gag-imCherry
Plasmid#208882Purposecodes for Gag from HIV-1 with an internal mCherry fluorescent proteinDepositorArticleInsertHIV-1 Gag
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-miRFP670nano3
Plasmid#207691PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-miRFP670nano3 cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-miniTurbo-Blast
Plasmid#207714PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miniTurbo-2A-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pH6HTC_mmHUR-Halo
Plasmid#175311PurposeProduction of HaloTag fusion proteinDepositorInsertHUR (Elavl1 Mouse)
UseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BECN1
Plasmid#203568PurposeMammalian expression of BECN1DepositorInsertBECN1 (BECN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
mScarletI_MapTauC
Plasmid#112964PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorArticleInsertMapTauC
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-PXN
Plasmid#112957PurposeIn vivo visualization of focal adhesions (can be used for colocalization studies)DepositorArticleInsertPXN
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
EMTB-mTurquoise2
Plasmid#137803PurposeVisualization of microtubulesDepositorArticleInsertEnsconsin microtubule binding domain
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xmTurquoise2_UtrCH
Plasmid#112963PurposeIn vivo visualization of F-actin (can be used for colocalization studies)DepositorArticleInsertUtrCH
UseTags3x mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYDNgp
Plasmid#162453PurposePositive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.DepositorInsertAga2p-2Glinker-eUnaG2 (AGA2 )
UseShuttle vector bacteria/yeastTagsHA nad myc and eUnaG2 fluorescent proteinExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-nLuc
Plasmid#199756PurposeElongation reporter construct to quantify elongation of synYFP[TTG]; Control construct of elongation reporter to quantify elongation duration of n_CGA stallingDepositorInsertsynYFP[TTG/AGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only