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Plasmid#124048PurposeEncodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing sadCas9 fused to mRuby2 P2A mRuby2, and an sgRNA targeting pUAS expressing mAzamiGreen in a PiggyBac destination vectorDepositorInsertPB_pEF1a-tet3G-P2A-iRFP713_pEF1a-sadCAS9::mRuby2-P2A-mRuby2-SV40PA_mu6-sgUAS_pUAS-NLS::mAzamiGreen-rgPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK234_055
Plasmid#123920PurposeEncodes the spCas9 gRNA GFP dropout expression cassette (bovine u6 promoter and constant region 2) as a type 234 part to be used in the MTK systemDepositorInsertgRNA-GFPDROPOUT-for Sp Cas9 bu6_20N_cr2
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_046
Plasmid#123976PurposeEncodes the Cas9 Thal5.10-2_hg18 homology destination with Hygromycin resistance cassette GFP dropout destination vector as a type 0 part to be used in the MTK systemhDepositorInserthThal5.10-2_hg18 CAS9 Destination - HygroR
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
JPF0419v
Plasmid#124021PurposeEncodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vectorDepositorInsertPB_pCAG-Lyn-iRFP713_pCAG-NES-mAzamiGreen_pCAG-p38KTR-mCerulean_pCAG-H2B::mScarlet
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSIIneo-TRE-hPhyB621- mCherry-HRasCT
Plasmid#139482PurposeExpresses PhyB621-mCherry-HRasCT in mammalian cells.DepositorInsertPhyB621 (PHYB Mustard Weed)
UseLentiviralTagsmCherry-HRasCTExpressionMammalianMutationG229L and deletion of aa Y at position 235 in mch…PromoterTet responsible elementAvailable sinceApril 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHTSHP-Smt-IIShhN
Plasmid#121135PurposeExpresses N-terminal SUMO tagged II-ShhN in bacteriaDepositorInsertShh (Shh Mouse)
UseTagsHIS, HRV3C site, MBP, and Smt tagExpressionBacterialMutationAmino acid sequence from 26-195, with two extra i…PromotertacAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C
Plasmid#217983Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172CPromoterT7Available sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T160C
Plasmid#220233Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT160CPromoterT7Available sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(85-160)
Plasmid#217984Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-100)
Plasmid#217978Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) T2C, C90A, T198C
Plasmid#220235Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationT2C, C90A, T198CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) C90A, T172C, T259C
Plasmid#220234Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90A, T172C, T259CPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CREB(1-280) C90A
Plasmid#217982Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationC90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280) A50C,C90A
Plasmid#217985Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationA50C, C90APromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(1-280)
Plasmid#217981Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CREB(160-280)
Plasmid#217980Purposebacterial expression of CREB1 domainsDepositorInsertCREB1 (Creb1 Rat)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-Cepheid1s-ST-WPRE
Plasmid#214968PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted; for better photostabilityDepositorInsertCepheid1s-ST
UseAAVTagsExpressionMutationPromoterhuman SynapsinAvailable sinceMarch 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-353)GCN4-L-GFP
Plasmid#193714PurposeExpression of dimeric, GFP tagged, human kinesin-1 (KIF5B aa1-353)DepositorInserthsKIF5B(1-353)-GCN4-L-GFP (KIF5B Human, Synthetic)
UseTagsEGFPExpressionMammalianMutationhsKIF5B(aa1-353);PromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-353)-GCN4-L-mCherry
Plasmid#193715PurposeExpression of dimeric, mCherry tagged, human kinesin-1 (KIF5B aa1-353)DepositorInserthsKIF5B(1-353)-GCN4-L-mCherry (KIF5B Human, Synthetic)
UseTagsmCherryExpressionMammalianMutationhsKIF5B(aa1-353); mCherry: Met1 DeletedPromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hsKIF5B(1-560)Rigor-L-2xHA
Plasmid#193718PurposeExpression of HA tagged human kinesin-1 (KIF5B aa1-560) to anchor microtubulesDepositorInserthsKIF5B(1-560)Rigor-L-2xHA (KIF5B Human, Synthetic)
UseTags2xHAExpressionMammalianMutationhsKIF5B(aa1-560), RIGOR: G234APromoterChicken beta-actinAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only