1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. pOCC61

   Plasmid

   #118877

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   mGFP, HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFlp-Km

   Plasmid

   #222334

   Purpose

   flp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Inserts

   sacB (sacB B. subtilis)
   flp (flp Budding Yeast)
   cI (cI )
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   cl, flp, and sacB

   Available since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1

   Plasmid

   #89693

   Purpose

   Luciferase reporter plasmid targeted at csr-1 locus

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   gsy-1 promoter, luciferase
   

   Use

   Yeast episomal vector

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   gsy-1

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pILGFPE9

   Plasmid

   #83545

   Purpose

   Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TEF1 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pShuttle

   Plasmid

   #192871

   Purpose

   Gateway compatible vector with BsaI cloning site

   Depositor

   Thorsten Stiewe

   Article

   Merle et al Mol Cancer. 2022 Oct 3;21(1):191. doi: 1

   Type

   Empty backbone

   Use

   Gateway-compatible cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 12, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

   Plasmid

   #112922

   Purpose

   Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH-hSyn-NCreIntN-T2A-mTagBFP2

   Plasmid

   #160502

   Purpose

   Expresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applications

   Depositor

   Shawn Je

   Article

   Khoo et al Mol Brain. 2020 Jul 2;13(1):101. doi: 10

   Insert

   NCreIntN
   

   Use

   Cre/Lox and Lentiviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. p415-PtrpC-Cas9-TtrpC-CYC1t

   Plasmid

   #68059

   Purpose

   Express humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.

   Depositor

   Christian Hong

   Article

   Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

   Insert

   humanized Cas9
   

   Use

   CRISPR; N. crassa, fungi

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBS1ClacZ

   Plasmid

   #55171

   Purpose

   lacZ-reporter vector, integration at amyE, ampr, cmr

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Bacillus biobrick box

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Aug. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMSCV_Mock

    Plasmid

    #223565

    Purpose

    The plasmid is expressed in mammalian cells as a control.

    Depositor

    Hyun Woo Park

    Article

    Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBCW

    Plasmid

    #62658

    Purpose

    Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

    Depositor

    Masato Ohtsuka

    Article

    Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

    Insert

    PhiC31
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. NLS-HA-2xMCP-KRAB

    Plasmid

    #126589

    Purpose

    Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

    Depositor

    David Segal

    Article

    O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

    Insert

    2XMCP-KRAB
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    human ubiquitin C promoter

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS pEGFP-N3 (1299)

    Plasmid

    #62043

    Purpose

    mammalian expression of NLS-EGFP

    Depositor

    Eric Schirmer

    Article

    Zuleger et al Genome Biol. 2013 Feb 15;14(2):R14. doi:

    Insert

    SV40 NLS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pEM705 Gs

    Plasmid

    #109350

    Purpose

    Wildtype Gαs

    Depositor

    Robert Lucas

    Article

    Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

    Insert

    GαS (GNAS Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a(+)::NL3F10H

    Plasmid

    #141290

    Purpose

    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

    Depositor

    Andrew Millar

    Article

    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

    Insert

    NLUC3F10H
    

    Use

    Luciferase

    Tags

    Flag (x3) and His (x10)

    Expression

    Bacterial

    Mutation

    Engineered for high stability (t1/2 = 11.5 days a…

    Promoter

    promoter for bacteriophage T7 RNA polymerase

    Available since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDECKO_GFP

    Plasmid

    #72619

    Purpose

    Expresses two gRNAs targeting GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDECKO_Malat1_C

    Plasmid

    #72622

    Purpose

    Expresses two gRNAs targeting MALAT1 promoter

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

    Insert

    gRNAs toward Malat1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (gRNA1) and H1 (gRNA2)

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. EC71173

    Plasmid

    #154061

    Purpose

    Level 1, Position 2 Golden Gate vector. HspHv17::Cre-U5-Cre

    Depositor

    Cristobal Uauy

    Article

    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

    Insert

    Cre recombinase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Introduction of U5 intron sequences, to prevent p…

    Promoter

    HvHSP17

    Available since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. asPink chromoprotein promoter-less

    Plasmid

    #118005

    Purpose

    For course in "Synthetic Biology: A Lab Manual"

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    RBS, asPink
    

    Use

    Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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