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pBBK74 pHomL-RPL18Bp-Scarlet(rfp)-Link-SFT2-SSA1t-HomR (AmpR)

Plasmid

#179058

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SFT2, a marker for the late Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SFT2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK73 pHomL-RPL18Bp-Scarlet(rfp)-Link-SED5-SSA1t-HomR (AmpR)

Plasmid

#179057

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SED5, a marker for the early Golgi

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SED5

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK72 pHomL-RPL18Bp-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

Plasmid

#179056

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-SCS2tmh, a marker for the endoplasmic reticulum

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-SCS2tmh

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK71 pHomL-RPL18Bp-ARC35-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179055

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of ARC35-Link-Scarlet(rfp), a marker for the actin cytoskeleton

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-ARC35-Link-Scarlet(rfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK69 pHomL-RPL18Bp-SPC42-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

Plasmid

#179053

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Scarlet(rfp), a marker for the spindle pole

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-SPC42-Link-Scarlet(rfp)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK 3XFLAG-MKK4-EE-NLS hygro

Plasmid

#87779

Purpose

Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK4

Use

Lentiviral

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Promoter

Available since

March 31, 2017

Availability

Academic Institutions and Nonprofits only
pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)

Plasmid

#179033

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubules

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Neon(gfp)-Link-TUB1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro-NFLAG TRF1

Plasmid

#16277

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

aa2-439

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
Flag FoxO3 NT

Plasmid

#14938

Depositor

Joan Massague

Article

Seoane et al Cell. 2004 Apr 16. 117(2):211-23.

Insert

FoxO3 NT (FOXO3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 1-249.

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
pWPI-TFDP1

Plasmid

#114299

Purpose

Constitutive expression of the TFDP1 protein

Depositor

Patrick Salmon

Article

Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

Insert

TFDP1 (TFDP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(2,100,50)-GFP

Plasmid

#124622

Purpose

Expresses adRNA(2,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(2,100,50)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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GluR2_adRNA(0,100,50)-GFP

Plasmid

#124623

Purpose

Expresses adRNA(0,100,50) targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

GluR2_adRNA(0,100,50)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRFP shRNA-resistant TrkB WT

Plasmid

#72714

Purpose

Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

Depositor

Freddy Jeanneteau

Article

Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

Insert

TrkB

Use

Tags

RFP

Expression

Mammalian

Mutation

shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

Promoter

CMV

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
MS2_adRNA

Plasmid

#124624

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with GFP

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MS2_adRNA_RAB7A

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-ARF4a

Plasmid

#67497

Purpose

Expression of shRNA targeting human ARF4a

Depositor

Richard Kahn

Article

Volpicelli-Daley et al Mol Biol Cell. 2005 Oct;16(10):4495-508.

Insert

HsARF4 shRNA (ARF4 Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pTX1-EV71-SL2

Plasmid

#126043

Purpose

In-vitro-transcription of Enterovirus 71 5UTR-SL2 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

Enterovirus 71 5'UTR SL2

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR1 DD_NLS

Plasmid

#124625

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR1 DD_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pTX1-SMN1-exon7-ESE1

Plasmid

#126041

Purpose

In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

Depositor

Frédéric Allain

Article

Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

Insert

SMN1 exon7 ESE1

Use

Tags

Expression

Mutation

Promoter

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGSTag Flag p38 gamma

Plasmid

#20818

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pGSTag Flag p38 gamma (Mapk12 Rat)

Use

Tags

Flag

Expression

Bacterial

Mutation

Promoter

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pLPC-NFLAG TRF1 deltaM

Plasmid

#16064

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Deleted myb domain (aa379-439)

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only