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Plasmid#216227PurposeTDP-43 with a C-terminal mCerulean tag for expression in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseTagsmCeruleanExpressionMammalianMutationPromoterCMVAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
MBD-IFP2.0C
Plasmid#215748PurposeExpresses MBD1 MBD domain fused to C-terminal part of IFP2.0 for 5mC detection in BiAD sensorsDepositorInsertIFP2.0C
UseUnspecifiedTagsExpressionMutationPromoterCMVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFv-IFP2.0N
Plasmid#215746PurposeExpresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0DepositorInsertIFP2.0N
UseTagsExpressionMammalianMutationPromoterSFFVAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_Zeo_3xSH2(GRB2)-CTEV-2xHA
Plasmid#214614Purposeadapter for RTK split TEV recruitment assaysDepositorInsert3xSH2 domains of GRB2-CTEV (GRB2 Human)
UseTags2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_ERBB4-NTEV-tcs-GV-2xHA
Plasmid#214613PurposeERBB4 receptor for split TEV assayDepositorInsertERBB4-NTEV (ERBB4 Human)
UseTagsTEV-Gal4-VP16-2xHAExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPI230
Plasmid#207431PurposeSafe haven knock-in plasmid for the act5 locus for C-terminal mNeonGreen fusionsDepositorInsertC-terminal mNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPI231
Plasmid#207432PurposeSafe haven knock-in plasmid for the act5 locus for N-terminal mNeonGreen fusionsDepositorInsertN-terminal mNeonGreen
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2084
Plasmid#137726PurposeGateway vector for inducible stem loop RNAi in T.b. brucei 2T1DepositorTypeEmpty backboneUseRNAi; Trypanosoma brucei tet inducible stem loopTagsExpressionMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-Cas9-sgHPRT1
Plasmid#196713PurposeCRISPR-KO. WT-SpCas9 and sgRNA targeting HPRT1. Editing-competent cells can be selected with 6-TGDepositorInsertCas9-T2A-BSD-U6-sgHPRT1
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterEF1a/hU6Available sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-CcuCas9
Plasmid#192127PurposeExpresses CcuCas9DepositorInsertCcuCas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos3_X
Plasmid#147944PurposeMammalian Expression of HsNanos3DepositorInsertHsNanos3 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos3-F4A_X
Plasmid#147946PurposeMammalian Expression of HsNanos3-F4ADepositorInsertHsNanos3-F4A (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos3-W7E_X
Plasmid#147947PurposeMammalian Expression of HsNanos3-W7EDepositorInsertHsNanos3-W7E (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNanos3-Y10E_X
Plasmid#147948PurposeMammalian Expression of HsNanos3-Y10EDepositorInsertHsNanos3-Y10E (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5_267-1017-V5-SBP-MBP_V
Plasmid#147762PurposeMammalian Expression of HsSmg5_267-1017DepositorInsertHsSmg5_267-1017 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg5-V5-SBP-MBP_V
Plasmid#147768PurposeMammalian Expression of HsSmg5DepositorInsertHsSmg5 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation C969T compared to the sequenc…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-HsNanos3_4-20-Strep_W
Plasmid#147825PurposeBacterial Expression of HsNanos3_4-20DepositorInsertHsNanos3_4-20 (NANOS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-HsNanos3_4-20-Strep_W
Plasmid#147826PurposeBacterial Expression of HsNanos3_4-20DepositorInsertHsNanos3_4-20 (NANOS3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-del133-155_T
Plasmid#147553PurposeMammalian Expression of HsSmg5-del133-155DepositorInsertHsSmg5-del133-155 (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_1-633_T
Plasmid#147557PurposeMammalian Expression of HsSmg7_1-633DepositorInsertHsSmg7_1-633 (SMG7 Human)
UseTagsExpressionMammalianMutationtwo silent mutation G216A, A1521G compared to the…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsSmg7_1-633_U
Plasmid#147644PurposeMammalian Expression of HsSmg7_1-633DepositorInsertHsSmg7_1-633 (SMG7 Human)
UseTagsExpressionMammalianMutationthree silent mutation G216A, T1185C and A1521G co…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-lambdaN-HA-HsNanos3_P
Plasmid#147200PurposeMammalian Expression of HsNanos3DepositorInsertHsNanos3 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNanos3_P
Plasmid#147203PurposeMammalian Expression of HsNanos3DepositorInsertHsNanos3 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Hsp2Cas9
Plasmid#192126PurposeExpresses Hsp2Cas9DepositorInsertHsp2Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Hsp1Cas9
Plasmid#192125PurposeExpresses Hsp1Cas9DepositorInsertHsp1Cas9
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Ccu-sgRNA-SV40-puro
Plasmid#192130PurposeExpresses Ccu-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCcu-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Hsp1-sgRNA-SV40-puro
Plasmid#192129PurposeExpresses Hsp1-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertHsp1-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSK-mU6-Cj-sgRNA-SV40-puro
Plasmid#192128PurposeExpresses Cj-SgRNA scaffold cloned with BbsI and puromycin resistance geneDepositorInsertCj-SgRNA scaffold
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hMYOD1 gRNA_1-MS2-Puro
Plasmid#192673PurposeLentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcriptionDepositorInsertHuman MYOD1 activating gRNA #1 (MYOD1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsNanos3_X
Plasmid#147962PurposeMammalian Expression of HsNanos3DepositorInsertHsNanos3 (NANOS3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 - TRC mTagBFP2-Synapsin1a
Plasmid#191567PurposeExpresses an shRNA and mTagBFP2-synapsin1aDepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNAAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
ppwd1.473.646.W601A
Plasmid#137660PurposeExpresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.DepositorInsertppwd1 (PPWD1 Human)
UseTags(His)6-thrombinExpressionBacterialMutation473-646-W601APromoterAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-G120E_W
Plasmid#147819PurposeMammalian Expression of HsSmg5-G120EDepositorInsertHsSmg5-G120E (SMG5 Human)
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg7_633-1091_T
Plasmid#147558PurposeMammalian Expression of HsSmg7_633-1091DepositorInsertHsSmg7_633-1091 (SMG7 Human)
UseTagsExpressionMammalianMutationone non silent mutation V854I compared to the seq…PromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
gMUM2-Citrine
Plasmid#181965PurposeMUM2 protein fused to Citrine, under control of native promoterDepositorInsertMUM2 (MUM2 Mustard Weed)
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM2Available sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
MUM2SP-Citrine
Plasmid#181963PurposeMUM2 signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertMUM2 signal peptide, fused to the Citrine YFP in pAD
UseTagsCitrine YFPExpressionPlantMutationPromoterMUM4pro 1.5kb fragmentAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ecm2 1-197
Plasmid#169924PurposeWT Ecm2 mutated to introduce stop codon after AA 197 of Ecm2DepositorInsertEcm2 1-197
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2xVlk1-3xFlag
Plasmid#172773PurposemRNA synthesis for injection into embryosDepositorInsertVlk1 (pkdcc.1.S Frog)
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB1
Plasmid#168800PurposeFluorogen activating protein (FAP) DiB1DepositorInsertFluorogen activating protein DiB1
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAD-DiB3
Plasmid#168802PurposeFluorogen activating protein (FAP) DiB3DepositorInsertFluorogen activating protein DiB3
UseTagsHis-tagExpressionBacterialMutationPromoteraraBADAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only