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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179012PurposeParent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genesDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK24 pCAS-Tyr-[gRNA: 7=HIS3] (SplitHygR, AmpR)
Plasmid#179008PurposeSp.Cas9 and gRNA yeast expression vector with HIS3 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Tet1
Plasmid#60938PurposeExpresses Tet1 in mammalian cellsDepositorInsertTen-Eleven Translocation 1 (Tet1 Mouse)
UseTagsflagExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
phage UbiC tagRFP-T-DDX6
Plasmid#119947PurposeLentiviral vector expressing tagRFP-T-DDX6 fusion proteinDepositorInsertDDX6 (DDX6 Human)
UseLentiviralTagstagRFP-TExpressionBacterial and MammalianMutationPromoterUbiCAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^Halo
Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorInsertVPS13A (VPS13A Human)
UseTagsHalo tag inserted at position 1372ExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHW110035
Plasmid#222444PurposeLevel 0, N-terminal tag module (CCAT-AATG) containing LoxP-mCherry-stop.DepositorInsertLoxP-mCherry-stop
UseSynthetic BiologyTagsExpressionPlantMutationnoPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pMBP-FnCas12a
Plasmid#113432PurposeExpresses 10xHis-MBP fused to FnCas12aDepositorInsertFnCas12a
UseTagsMBPExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
lenti-pegRNA-puro backbone
Plasmid#214085PurposeLentiviral backbone plasmid to insert pegRNAs of interest. The cloning site is BsmbI.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterhU6, EF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCS-BioID2-HA
Plasmid#74224PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tagDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDY1173_ADAR1p150
Plasmid#193192PurposeExpress full length ADAR1p150DepositorInsertADAR1p150 (ADAR Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFB1
Plasmid#116799PurposeLentiviral expression of TGFB1DepositorInsertTGFB1 (TGFB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)
Plasmid#179002PurposeSp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFLP-hyg
Plasmid#87831PurposeExpresses FLP recombinase with hyg selection markerDepositorInserthyg
UseTagsNoneExpressionBacterialMutationNonePromoterAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_ mito_roGFP2-Orp1
Plasmid#155042PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsmitochondrial targeting sequence and roGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-hygro-STING
Plasmid#102598PurposeExpress STING for IFN-beta luciferase reporter assay.DepositorInsertSTING (STING1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV SCH9
Plasmid#212708PurposeA SCHEMA evolved AAV variant effective at targeting subventricular NSCsDepositorInsertAAV2 Rep SCH9 Cap
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-JEDI-1P-Kv-WPRE
Plasmid#202612PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSynDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
eBFP2 (eBFP2)_CD
Plasmid#66034PurposeMoClo Basic Part: CDS - Fluorescent protein. Blue. Derived from Addgene#14891. [C:eBFP2:D]DepositorInsertFluorescent reporter - BFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
lenti-EF1a-dCas9-VPR-Puro
Plasmid#99373Purpose3rd generation lenti vector encoding dCas9 (S. pyogenes) fused with VP64-p65-Rta (VPR) and 2A puromycin resistance marker; EF1a-dCas9-VPR-P2A-Puro-WPRE)DepositorInsert(Sp)dCas9-VPR-P2A-Puro
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 Lysosomal-METRIQ
Plasmid#135401PurposeExpresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activityDepositorInsertDNAse II alpha (DNASE2 Human)
UseLentiviralTagsT2A, mCherry, and sfGFPExpressionMammalianMutationSingle-nucleotide polymorphism C432TPromoterAvailable sinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNDL-54
Plasmid#161715PurposeIn vitro transcription construct for production of membrane acvr1b-Cry2 mRNADepositorInsertMyr-acvr1b-Cry2 (acvr1ba Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNDL-60
Plasmid#161720PurposeIn vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNADepositorInsertcytosolic acvr2b-CIB1N (acvr2b Zebrafish)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Fos-CreERT2
Plasmid#194643PurposeExpresses Cre-ERT2 under the control of c-Fos promoterDepositorInsertFos-CreERT2
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-Bxb1o_nlsFlag
Plasmid#119901PurposeExpresses Bxb1 integrase in mammalian cellsDepositorInsertMammalian codon optimized Bxb1 integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF2158
Plasmid#141949PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertBCL11B (BCL11B Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-TPD54
Plasmid#172449PurposeExpression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry tagDepositorInsertTPD54 (TPD52L2 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
CAG-NLS-GFP
Plasmid#104061PurposeAAV expression of nuclear localized GFP with SV40 NLSDepositorHas ServiceAAV PHP.eBInsertGFP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceAug. 31, 2018AvailabilityAcademic Institutions and Nonprofits only
