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pMRX-No-HaloTag7-mGFP-LC3

Plasmid

#184901

Purpose

Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

Depositor

Noboru Mizushima

Article

Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

Insert

LC3B

Use

Tags

HaloTag7 and monomeric EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-NFIA

Plasmid

#141403

Purpose

Inducible Expression of NFIA

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

NFIA (NFIA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDDGFP_LEU2D

Plasmid

#58352

Purpose

Used for expressing recombinant proteins as a C terminal GFP-His tagged constuct in S.cerevisiae

Depositor

Simon Newstead

Article

Parker et al Protein Sci. 2014 Jun 20. doi: 10.1002/p

Type

Empty backbone

Use

Tags

GFP-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_WT USP7

Plasmid

#131242

Purpose

Mammalian expression of N-terminally Myc-tagged USP7

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV enhancer + CMV promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAM5505

Plasmid

#132664

Purpose

pRL623-based helper plasmid for conjugations, with RSF1010 mobA gene

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Insert

MobA

Use

Tags

Expression

Bacterial

Mutation

please see depositor comments below

Promoter

p1,p3

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEB1-mVenus NB

Plasmid

#103986

Purpose

Plasmid encoding mVenus NB

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mVenus NB

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, F64L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Use

Tags

pmRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCI-EGFP-NR1 wt

Plasmid

#45446

Depositor

Robert Malinow, Andres Barria

Article

Barria et al Neuron. 2002 Jul 18;35(2):345-53.

Insert

NR1 (Grin1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

EGFP inserted after the predicted signal peptide …

Promoter

CMV

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE_ENTR_12GLI-FLuc_R4-R3

Plasmid

#113712

Purpose

Single pathway reporter; hedgehog promoter-driven expression of firefly luciferase; MultiSite Gateway entry vector.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Insert

12GLI-FLuc

Use

Mule gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKIIa-mCherry

Plasmid

#114469

Purpose

mCherry Control

Depositor

Karl Deisseroth

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

CaMKIIa

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-NL63-D14

Plasmid

#172666

Purpose

encodes NL63 spike protein, for pseudovirus production

Depositor

David Nemazee

Article

Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

Insert

HCNV63gp2 (HCNV63gp2 human coronavirus NL63)

Use

Lentiviral and Retroviral

Tags

NA

Expression

Mammalian

Mutation

c-terminal deletion of 14aa

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-Cas9-2A-GFP-sgRNA

Plasmid

#124889

Purpose

Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

Depositor

Harvey Lodish

Article

Li et al Haematologica. 2016 Jun;101(6):e216-9. d

Insert

Cas9

Use

Retroviral

Tags

2A-GFP

Expression

Mutation

Promoter

MSCV

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP53

Plasmid

#22606

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP53 (USP53 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 65 aa

Promoter

CMV/TO

Available since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pJR101

Plasmid

#187241

Purpose

Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

Depositor

Marco Jost, Jonathan Weissman

Article

Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

Insert

Lentiviral sgRNA vector for Perturb-seq with mU6 promoter

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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His-GFP SNAP25

Plasmid

#170872

Purpose

Mammalian expression of His-GFP SNAP25.

Depositor

Justin Taraska

Article

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

SNAP 25 (Snap25 Rat)

Use

Tags

(His)6 and EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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Lifeact-mCherry

Plasmid

#193300

Purpose

mammalian transient expression - expression of Lifeact-mCherry

Depositor

Klaus Hahn

Article

Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

Insert

Lifeact

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1

Plasmid

#121478

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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