-
Plasmid#188055PurposeExpression of BLZinCh-P20, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cellsDepositorInsertBLZinCh-P20
UseTagsExpressionMammalianMutationPromoterCMV promotorAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BLZinCh-P30
Plasmid#188056PurposeExpression of BLZinCh-P30, a dual read-out BRET-FRET sensor for intracellular Zn2+ imaging, in mammalian cellsDepositorInsertBLZinCh-P30
UseTagsExpressionMammalianMutationPromoterCMV promotorAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SUN1 GFP
Plasmid#187682PurposeIn vivo visualization of SUN1DepositorInsertSUN1 (Sun1 Mouse)
UseTagsEGFPExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YE1
Plasmid#154145PurposeExpresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.DepositorInsertdLbCpf1-BE-YE1 (dCas12a-BE-YE1)
UseTagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)PromoterCMVAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YE2
Plasmid#154146PurposeExpresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.DepositorInsertdLbCpf1-BE-YE2 (dCas12a-BE-YE2)
UseTagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)PromoterCMVAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-dLbCpf1-BE-YEE
Plasmid#154148PurposeExpresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.DepositorInsertdLbCpf1-BE-YEE (dCas12a-BE-YEE)
UseTagsSV40 NLSExpressionMammalianMutationAPOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…PromoterCMVAvailable sinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐NGA
Plasmid#187059PurposeABEmax-SpCas9-NGX‐NGADepositorInsertABEmaxNGX‐NGA
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐NGC
Plasmid#187060PurposeABEmax-SpCas9-NGX-NGCDepositorInsertABEmaxNGX‐NGC
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV‐ABEmaxNGX‐loop
Plasmid#187061PurposeABEmax-SpCas9-NGX-loopDepositorInsertABEmaxNGX‐loop
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3×FLAG-SLC35A2
Plasmid#186283Purposetransient expression of N-terminal 3×FLAG tagged SLC35A2 isoform c/UGT1 in mammalian cellsDepositorInsertSLC35A2 (SLC35A2 Human)
UseTags3×FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K468TAG)-FLAG
Plasmid#182658PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(K211TAG)-FLAG
Plasmid#182655PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K211 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK211TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_NFL(R438TAG)-FLAG
Plasmid#182657PurposeExpresses mouse neurofilament light chain with a TAG codon at the position R438 and a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationR438TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(R438TAG)
Plasmid#182662PurposeExpresses mouse neurofilament light chain with a TAG codon at the position R438 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationR438TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K363TAG)
Plasmid#182661PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K363 & an N-terminal FLAG tag (DYKDDDDK) in mammalian cells. Can be used for genetic code expansion & click labeling of NFL.DepositorInsertmouse neurofilament light chain with a K363TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK363TAGPromoterCMVAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_FLAG-NFL(K468TAG)
Plasmid#182663PurposeExpresses mouse neurofilament light chain with a TAG codon at the position K468 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.DepositorInsertmouse neurofilament light chain with a K468TAG mutation (Nefl Mouse)
UseTagsFLAG tag (DYKDDDDK)ExpressionMammalianMutationK468TAGPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 S111F-msfGFP
Plasmid#180340Purposemammalian expression of human SEPT9_i1 S111F fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 S111FPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 R106W-msfGFP
Plasmid#180339Purposemammalian expression of human SEPT9_i1 R106W fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 R106WPromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 NCmut1-msfGFP
Plasmid#180329Purposemammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 R289A R290A K291A K294APromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1 D10-25-msfGFP
Plasmid#180334Purposemammalian expression of human SEPT9_i1 D10-25 fused to monomeric superfolder GFPDepositorInsertSEPTIN9_v1 (SEPTIN9 Human)
UseTagsmsfGFPExpressionMammalianMutationSEPT9_i1 D10-25PromoterCMVAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only