-
Plasmid#118405PurposecpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase A activity.DepositorInsertExRai-AKAR
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUDE1084
Plasmid#168500Purposeexpression of T7RNA polymerase and CRISPR endonunclease Fncas12aDepositorInsertsFncas12a
T7RNA polymerase
UseCRISPRTagsExpressionMutationPromoterTDH3 and TEF1Available sinceDec. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRedF
Plasmid#118057PurposeGBPart - RedF Luciferase CDSDepositorInsertRed Firefly Luciferase CDS
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationRed mutant S286YPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSLQ5135: pCAG-BFP-GuideArray
Plasmid#210606PurposeGuide array for hyperdCas12a-based transcriptional activator with AAAT synSeparatorDepositorInsertpCAG-BFP-GuideArray
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNLuc
Plasmid#118058PurposeGBPart - NLuc Luciferase CDSDepositorInsertNano Luciferase
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGrRenilla
Plasmid#118060PurposeGBPart - GreenRenilla Luciferase CDSDepositorInsertGreen Renilla
UseLuciferase and Synthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ167
Plasmid#69309PurposeGateway entry vector with Cas9pDepositorInsertCas9p (Plant codon-optimized; high GC content at 5 prime region)
UseCRISPRTagsExpressionMutationNLS fusionPromoterAvailable sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pbGH
Plasmid#118061PurposeGBPart - Bovine growth hormone terminatorDepositorInsertbgH_poly(A)
UseSynthetic Biology; GbpartTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pC0059 PbuCas13 (B03) His6-TwinStrep-SUMO-BsaI
Plasmid#115209PurposeExpresses PbuCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPbuCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2xp53_RE
Plasmid#118053PurposeGBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPomyces-1
Plasmid#61736PurposeStreptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNADepositorInsertssSpCas9
tracrRNA
crRNA cassette
UseCRISPRTagsExpressionBacterialMutationBbsI-flanked lacZ cassette inserted in place of s…Promotergapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsIAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorInsertLifeact-HaloTag11 and Tomm20-HaloTag7
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC0065 Pin2Cas13 (B09) His6-TwinStrep-SUMO-BsaI
Plasmid#115216PurposeExpresses Pin2Cas13b from a SUMO-tagged bacterial expression constructDepositorInsertPin2Cas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ152
Plasmid#69303PurposeGateway entry vector with pco-dCas9-VP64DepositorInsertpco-dCas9-VP64 (Plant codon-optimized)
UseCRISPRTagsExpressionMutationVP64 activator fusion; NLS fusionPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
3xDBE_RE::Renilla
Plasmid#124535PurposeFoxo Renilla luciferase reporterDepositorInsertFOXO Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC0058 PinCas13 (B02) His6-TwinStrep-SUMO-BsaI
Plasmid#115208PurposeExpresses PinCas13b from a SUMO-tagged bacterial expression constructDepositorInsertPinCas13
UseTagsSUMOExpressionBacterialMutationPromoterAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only