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pLENTI_RPS3_K227/230R_Keima

Plasmid

#127146

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

RPS3_Keima (RPS3 Human)

Use

Lentiviral

Tags

Expression

Mutation

K227R/K230R

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_UNK_G1

Plasmid

#127118

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA UNK (UNK Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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KHBD00582

Plasmid

#39666

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5369 (CG5369 Fly)

Use

Gateway donr vector

Tags

Expression

Mutation

Promoter

none

Available since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
V405 4HA-CKIe

Plasmid

#13724

Depositor

David Virshup

Article

Rivers et al J Biol Chem. 1998 Jun 26. 273(26):15980-

Insert

casein kinase 1 epsilon (CSNK1E Human)

Use

Tags

4 HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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KHBD00222

Plasmid

#34370

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG1832 (CG1832 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCR3-FLAG-TRAF6-C70A

Plasmid

#43926

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 Mouse)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

C70A

Promoter

Available since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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SuperNova/pRSETB

Plasmid

#53234

Purpose

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Takeharu Nagai

Article

Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

Insert

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Use

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag p38 gamma

Plasmid

#20353

Depositor

Roger Davis

Article

Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

Insert

pDCNA3 Flag p38 gamma (Mapk12 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX5X.1 PINK1 WT

Plasmid

#13321

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 kinase domain (PINK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acids 112-496

Promoter

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMTH PKC zeta

Plasmid

#8414

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C zeta (Prkcz Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
KHBD00233

Plasmid

#34377

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7803 (z Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pEBTetD-β-tubulin_SNAP

Plasmid

#136832

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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mt deltaN20 TFB2MdeltaC3

Plasmid

#205062

Purpose

TFB2M protein expression

Depositor

Smita Patel

Article

Basu et al Nucleic Acids Res. 2020 Mar 18;48(5):260

Insert

TFB2M (TFB2M Human)

Use

Tags

Expression

Bacterial

Mutation

Lacks N-terminal 20 aa and C-terminal 3 aa

Promoter

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7b1

Plasmid

#14622

Depositor

Roger Davis

Article

Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

Insert

MKK7b1 (Map2k7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_RACK1

Plasmid

#127123

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RACK1 (RACK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Lentiviral CaMKKbeta

Plasmid

#33322

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

human PGK

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-Centrin3

Plasmid

#136806

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin3

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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Erk1

Plasmid

#29578

Depositor

Dustin Maly

Article

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Erk1 (MAPK3 Human)

Use

Tags

FLAG, HIS6, and TEV

Expression

Bacterial

Mutation

K289N mutation present. Starting Met removed from…

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only