-
Plasmid#1186DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only
These results may also be relevant.
-
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC65
Plasmid#118865Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
UseTagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
UseTagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS1C
Plasmid#55168PurposeEmpty vector, integration at amyE, ampr, cmrDepositorTypeEmpty backboneUseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFlp-Km
Plasmid#222334Purposeflp-cl-sacB-pL1V-Lv1-neo-pBBR1-ELT3DepositorUseTagsExpressionMutationPromotercl, flp, and sacBAvailable sinceAug. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC gRNA Shuttle
Plasmid#47024PurposeEncodes a template from which gRNAs can be made via InFusion cloning. The Medicago truncatula U6.6 promoter drives the gRNA. For use in plants.DepositorInsertgRNA Shuttle
UseCRISPR; Cas9TagsExpressionPlantMutationG to T cloning mutation at position 323PromoterMedicago truncatula U6.6Available sinceSept. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pShuttle
Plasmid#192871PurposeGateway compatible vector with BsaI cloning siteDepositorTypeEmpty backboneUseGateway-compatible cloning vectorTagsExpressionMutationPromoterAvailable sinceDec. 12, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1
Plasmid#89693PurposeLuciferase reporter plasmid targeted at csr-1 locusDepositorInsertgsy-1 promoter, luciferase
UseYeast episomal vectorTagsluciferaseExpressionMutationPromotergsy-1Available sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKESaPar
Plasmid#211829PurposeLow copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-hSyn-NCreIntN-T2A-mTagBFP2
Plasmid#160502PurposeExpresses NCreIntN in neurons in split intein-mediated split-Cre recombinase applicationsDepositorInsertNCreIntN
UseCre/Lox and LentiviralTagsmTagBFP2ExpressionMammalianMutationPromoterhSynAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pveg
Plasmid#55173PurposeVery strong constitutive promoterDepositorInsertveg promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromotervegAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
ePPEplus
Plasmid#205241PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertc-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRTagsExpressionPlantMutationDetailed in manuscriptPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIW601-KmCRISPR
Plasmid#98907PurposeK. marxianus CRISPR Plasmid for sgRNA cloningDepositorInsertsCodon optimized Cas9
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsSV40ExpressionYeastMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiTagsExpressionYeastMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112922PurposeGateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UseGateway entry vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKERepPar
Plasmid#211827PurposeMedium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.DepositorInserteGFP
UseTagsExpressionBacterialMutationPromoterT5Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only