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pGEX-Pak1 83-149

Plasmid

#12216

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (Pak1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pCMV5-rat ERK2-D319N

Plasmid

#40820

Depositor

Natalie Ahn

Article

Emrick et al J Biol Chem. 2001 Dec 7;276(49):46469-79

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

D319N

Promoter

CMV

Available since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only
pCDNA3.1 AT1AR-TSTS/4A

Plasmid

#45634

Depositor

Robert Lefkowitz

Article

Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

Insert

AT1AR-TSTS/A (Agtr1a Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Thr332, Ser335, Thr336, and Ser338 all changed to…

Promoter

CMV

Available since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only
pCMV-dCpf1-BE0

Plasmid

#107678

Purpose

Expresses dLbCpf1-BE0 in mammalian cells

Depositor

Jia Chen

Article

Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Insert

dLbCpf1-BE0

Use

Tags

Expression

Mammalian

Mutation

D832A/E925/D1148A

Promoter

CMV

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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Syt2

Plasmid

#186688

Purpose

Expresses Synaptotagmin 2 in E. Coli

Depositor

Axel Brunger

Article

Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

Insert

Synaptotagmin 2

Use

Tags

GST and thrombin site

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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p130Cas

Plasmid

#186131

Purpose

For expression of recombinant p130Cas in SF9 cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

BCAR1 (Bcar1 Rat)

Use

Tags

His6

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1 E63A

Plasmid

#112861

Purpose

plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

APOBEC1 (Apobec1 Rat)

Use

Tags

Expression

Mammalian

Mutation

changed Glutamic acid 63 to Alanine

Promoter

CMV promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pCUP1-NMGR

Plasmid

#1211

Depositor

Susan Lindquist

Article

Li et al Science 2000 Jan 28;287(5453):661-4.

Insert

sup35 NM domain fused with GR (SUP35, Nr3c1 Rat, Budding Yeast)

Use

Tags

GR

Expression

Yeast

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pORANGE GSG1l-GFP KI

Plasmid

#131492

Purpose

Endogenous tagging of GSG1-l: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gsg1l Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22, G120A)

Plasmid

#45449

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120, Glycine at position 12…

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pCI-syn-fRGECO2

Plasmid

#125244

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

Expression

Mammalian

Mutation

W44Y, D432A

Promoter

synapsin

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 epsilon K49E

Plasmid

#11943

Depositor

Michael Yaffe

Article

Brunet et al J Cell Biol. 2002 Mar 4. 156(5):817-28.

Insert

Ywhae (Ywhae Rat)

Use

Tags

His

Expression

Mammalian

Mutation

K49E

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
Syn2ApcDNA4/TO

Plasmid

#14817

Depositor

Thomas Weimbs

Article

Low et al Dev Cell. 2003 May . 4(5):753-9.

Insert

Syntaxin2A (Stx2 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2007

Availability

Academic Institutions and Nonprofits only

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EBFP2-CathespinB-6

Plasmid

#55236

Purpose

Localization: Lysosomes, Excitation: 383, Emission: 448

Depositor

Michael Davidson

Article

Sundaresan et al J Clin Oncol. 1987 Nov;5(11):1739-45.

Insert

CathespinB

Use

Tags

EBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

Plasmid

#179073

Purpose

Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

TDH3p-Scarlet(rfp)-Link-2xPH

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK75 pHomL-RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)-SSA1t-HomR (AmpR)

Plasmid

#179059

Purpose

Vector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-2xPH(PLCd), a marker for the plasma membrane

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

RPL18Bp-Scarlet(rfp)-Link-2xPH(PLCd)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin G

Plasmid

#68817

Purpose

Geph G domain expression in mamalian cells

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

NIL

Promoter

CMV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only
F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Promoter

Available since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
mEos2-Homer1-N-18

Plasmid

#57388

Purpose

Localization: Membrane Adaptor, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Homer1 (Homer1 Rat)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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