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AAV-CAG-hChR2-H134R-tdTomato

Plasmid

#28017

Purpose

AAV-mediated expression of hChR2(H134R) for neuronal excitation.

Depositor

Karel Svoboda

Article

Mao et al Neuron. 2011 Oct 6;72(1):111-23.

Has Service

AAV Retrograde

Insert

humanized ChR2-H134R-tdTomato

Use

AAV; Adeno associate viral

Tags

tdTomato

Expression

Mutation

H134R

Promoter

CAG

Available since

Nov. 30, 2011

Availability

Academic Institutions and Nonprofits only

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1245 pCMVb p300

Plasmid

#10717

Depositor

William Sellers

Insert

p300 (EP300 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pCLBW cox8 EGFP mCherry

Plasmid

#78520

Purpose

Fluorescent mitophagy reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

none

Use

Retroviral

Tags

cox8 EGFP mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8s

Plasmid

#162371

Purpose

Mammalian expression of ultrafast protein calcium sensor

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Insert

jGCaMP8s

Use

Tags

6xHis

Expression

Mammalian

Mutation

S26M F286Y Q315H

Promoter

CMV

Available since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Plasmid

#209723

Purpose

Expresses mitochondrially targeted low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPB9v1

Plasmid

#210028

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
rtta-T2A-puro

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Mutation

Promoter

EF-1α and TRE3G

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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XLone-GFP

Plasmid

#96930

Purpose

Tunable and temporal expression control of GFP

Depositor

Xiaojun Lian

Article

Randolph et al Sci Rep. 2017 May 8;7(1):1549. doi: 10.1

Insert

Green Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-mCherry

Plasmid

#197420

Purpose

Cas9 from S. pyogenes with 2A-mCherry, and cloning backbone for sgRNA

Depositor

Torsten Wittmann

Article

Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

Insert

Cas9

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Mfn1

Plasmid

#141154

Purpose

Mammalian expression and labeling mitofusin 1

Depositor

Gia Voeltz

Article

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Insert

Mitofusin 1 (MFN1 Human)

Use

Tags

AcGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pKLV-EF1a-mCherryLuciferase-W

Plasmid

#200101

Purpose

Lentiviral vector expressing mCherry fused with Luciferase at the C-terminus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Luciferase

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Hygro-PIP-FUCCI

Plasmid

#138714

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Mutation

Promoter

hPGK

Available since

May 8, 2020

Availability

Academic Institutions and Nonprofits only

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pET-NLS-Cas9-6xHis

Plasmid

#62934

Purpose

Expression of NLS-Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

NLS-Cas9-6xHis

Use

Tags

6xHis tag and NLS

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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hprtSAGFP

Plasmid

#41594

Purpose

In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

Depositor

Maria Jasin

Article

Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

Insert

SA-GFP reporter

Use

Mouse Targeting; Dsb repair reporter substrate

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_OCT4

Plasmid

#26816

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Sep 29. ():.

Insert

5'UTR-OCT4-3'UTR (POU5F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pHCMV-EcoEnv

Plasmid

#15802

Depositor

Miguel Sena-Esteves

Article

Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

Insert

MLV env (ecotropic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Neo

Plasmid

#139449

Purpose

Lentiviral vector with gRNA scaffold and neomycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

no sgRNA inserted; resistance gene: neoR

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a / U6

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pRDA_078

Plasmid

#158582

Purpose

Lentiviral expression of BE4max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Cas9 D10A

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pXJ197-pAAV-PB3'IR-U6-gRNA-directcapture-EF1a-GFP-KASH-PB5'IR

Plasmid

#210523

Purpose

AAV piggybac transposon vector, with GFP-KASH reporter and gRNA cloning site. Compatible with 10X 3' and 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

GFP-KASH
U6-gRNA-directcapture

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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