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Plasmid#36877DepositorInsertGFP
UseTagsExpressionMammalianMutationF64L; S65T; V163A; S175GPromoterCAG (chicken beta actin promoter and CMV enhancer)Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-eGFP-Esp3I
Plasmid#81226PurposeCloning plasmid derived from loxP reporter. The Kan and pA where digested and removed with XhoI/MluI and replaced with two Esp3I sitesDepositorInsertEsp31-Neo-Esp31 deletion cassette upstream of EGFP
UseTagsExpressionMammalianMutationPromoterpCBaAvailable sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HDAC4-IGd2
Plasmid#22536PurposeTo be used for the RNAi screening purposes (not for HDAC4 expression); see paperDepositorInsertHistone Deacetylase 4 (HDAC4 Human)
UseTagsIGd2 = Ires-destabilized GFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCAG-p21_T145A/S146A
Plasmid#42623DepositorInsertp21 (CDKN1A Human)
UseTagsExpressionMammalianMutationT145A/S146APromoterCAGAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
CpcBfull-NaGLS fusion_CpcA
Plasmid#74004PurposeExpresses the Nicotiana attenuata GLS as a fusion with the CpcB protein plus the CmR cassete in SynechocystisDepositorInsertNaGLS and CmR
UseTagsExpressionMutationPromotercpcAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Nat
Plasmid#232094PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-CMV-EGFPCAAX
Plasmid#232140PurposeExpresses EGFP with a CAAX motif under control of a CMV promoterDepositorInsertEGFPCAAX
UseZebrafish expressionTagsExpressionMutationPromoterCMVAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG-GluA2-Fab-LC
Plasmid#230063PurposeExpresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.DepositorInsertAnti-GluA2 Fab light chain fragment
UseTagsExpressionMammalianMutationPromoterChicken beta actin (CAG)Available sinceFeb. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Kan
Plasmid#232096PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRTagsExpressionYeastMutationPromoterZ3Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA
Plasmid#225350Purposepiggyback backbone for non-fluorescent GFP tether and H2B-IRFP expressionDepositorInsertH2B (H2B Human, Synthetic)
UseTagsmiRFP and non-fluorescent GFP (Y67F)-P2AExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 IST1 (1-366)
Plasmid#193039PurposeBacterial expression plasmid for full-length IST1 (1-366).DepositorInsertIST1 (IST1 Human)
UseTags6xHis-SumoExpressionBacterialMutationPromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-CAPN7-OSF
Plasmid#193041PurposeMammalian expression plasmid encoding full-length CAPN7 with C-terminal OSF tag (One-Strep-FLAG)DepositorInsertCAPN7 (CAPN7 Human)
UseTagsOSF (One-Strep-FLAG)ExpressionMammalianMutationPromoterChicken Beta-actinAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCA528 CAPN7 (81-165)
Plasmid#193032PurposeBacterial expression plasmid for CAPN7 (81-165) C-terminal MIT domainDepositorInsertCAPN7 (CAPN7 Human)
UseTags6xHis-SumoExpressionBacterialMutationResidues 81-165PromoterT7Available sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xControlgRNA
Plasmid#224567PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xControlgRNA
Plasmid#224568PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-memRFP-3xPax7gRNA
Plasmid#224569PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.DepositorInsertmRFP1
UseCRISPRTagsMembrane localization signalExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-nucGFP11-3xPax7gRNA
Plasmid#224570PurposeUbiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.DepositorInsertGFP11-H2B
UseCRISPRTagsHistone H2B (nuclear localization)ExpressionMutationCodon optimizedPromoterAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCambia3301-AtU6-MAR1
Plasmid#210757PurposeMutagenesis of the Arabidopsis MAR1 gene for efficient screening.DepositorInsertAtU6-26 pro::MAR1 sgRNA
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26Available sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-pCAG-mock
Plasmid#228504PurposeThis plasmid can be used for control experiments and can also be used as a plasmid backboneDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only