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Plasmid#175580PurposeExpression of CRISPR-associated transposasesDepositorInsertPtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)
UseTagsExpressionBacterialMutationNOPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL1119 (pDonor)
Plasmid#160732PurposeMini-transposon donor plasmid for V. cholerae CAST system. pUC19 vector backbone.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC57-CmYLCV
Plasmid#205245PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site
UseCRISPRTagsExpressionPlantMutationPromoterCmYLCVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pR0306 CcaCas13b His6-TwinStrep-SUMO
Plasmid#182687PurposeExpresses CcaCas13b with N terminal 6xHis-SUMO tag for recombinant bacterial expression and purificationDepositorInsertCcaCas13b
UseTagsSUMOExpressionBacterialMutationPromoterT7Available sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…TagsExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
HiFi-nCas9-NG_P2A-mCherry
Plasmid#219936PurposeMammalian expression of Sp-HiFi-nCas9-NG with P2A-mCherry marker for use with MOBE systemsDepositorInsertSp-HiFi-nCas9(D10A)-NG_P2A-mCherry
UseCRISPRTagsSV40 bpNLS and SV40 bpNLS-P2A-mCherryExpressionMammalianMutationHiFi-nCas9(D10A/R691A)PromoterCMVAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMPRAv3:minP-GFP
Plasmid#109036PurposeMPRA v3 minimal promoter with GFP donorDepositorInsertemGFP
UseTagsExpressionMammalianMutationPromoterminPAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO_TOMM20_HaloTag7
Plasmid#169330PurposeExpression of TOMM20-HaloTag7 in mammalian cellsDepositorInsertTOMM20-HaloTag7 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::FLuc
Plasmid#124531PurposeSMAD2 FLuc luciferase reporterDepositorInsertSMAD FLuc reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[BoBS]
Plasmid#68431PurposeTransient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6_(GLuc)_INT[3xMS2_SL]
Plasmid#68426PurposeTransient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoterDepositorInsertINT construct_bearing three MS2 Stem-loops
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhuman U6Available sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-VP64 dCas9 VP64-T2A-GFP
Plasmid#59791PurposeCo-expresses human optimized S. pyogenes dCas9 fused to two copies of VP64 and GFPDepositorInserthumanized VP64 dead Cas9 VP64 T2A GFP
UseCRISPR and LentiviralTagsFlagExpressionMammalianMutationD10A and H840APromoterAvailable sinceOct. 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hU6
Plasmid#102840PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_hU6promoter
UseTagsExpressionMutationPromoterhU6Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
LentiUniversal-Puro
Plasmid#127749PurposeLentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_VPR_yl
Plasmid#107677PurposeCRISPR-dCas9-VPR vector for Yarrowia lipolytica, expressing dCas9-VPR and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-VPR
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRGE32
Plasmid#63159PurposeExpress sgRNA/PTG with rice snoRNA U3 promoter and Cas9 with rice ubiquitin promoter for transient expression in protoplastDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRice snoRNA U3 promoter for gRNA/PTG expression a…Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pH2B-miRFP670nano
Plasmid#127438PurposeHistone 2B labeling with near-infrared fluorescent protein miRFP670nanoDepositorInsertH2B-miRFP670nano (H2BC11 Human, Synthetic)
UseTagsmiRFP670nanoExpressionMammalianMutationPromoterCMVAvailable sinceJuly 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A
Plasmid#205760PurposeSingle-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transitionDepositorInsertYFP-SLBP(18-126)-IRES-H1-Skylan62A
UseLentiviralTagsExpressionMammalianMutationwtPromoterAvailable sinceAug. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11296: pHR-HSP-hyperdCas12a-miniVPR-EPS-puro
Plasmid#210604Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
7xE-Box::Renilla
Plasmid#124532Purposec-Myc Renilla luciferase reporterDepositorInsertc-Myc Renilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only