-
Plasmid#56033PurposeLocalization: Nucleus/Centromeres, Excitation: 586, Emission: 632DepositorInsertCENPB (CENPB Human)
UseTagstdKatushka2ExpressionMammalianMutationaa 1-169 of CENPB (DNA Binding Domain)PromoterCMVAvailable sinceApril 28, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEMS2031
Plasmid#49134PurposeAAV plasmid with OLIG1 (Ple305) promoter driving expression of iCre.DepositorInsertssAAV-Ple305-iCre
UseAAVTagsExpressionMammalianMutationPromoterOLIG1Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
ALARM NMR
Plasmid#90209PurposeExpresses the human La antigen for use in ALARM NMRDepositorInsertLa antigen (SSB Human)
UseTags6xHisExpressionBacterialMutationcontains amino acids 100-324PromoterT7 promoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNS19-LV-mutRFP-2A-GFP
Plasmid#113716PurposeLentiviral vector for fluorescent reporter system: expresses a green fluorescent protein that is preceded by an inactive version of a red fluorescent protein (2 ntd substitution at the fluorophore)DepositorInsertmutRFP-2A-GFP-IRES-Puro
UseLentiviral; Reporter systemTagsExpressionMammalianMutationY64L (mutRFP)PromoterAvailable sinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS1996
Plasmid#49126PurposeAAV plasmid with UGT8 (Ple267) promoter driving expression of iCre.DepositorInsertssAAV-Ple267-iCre
UseAAVTagsExpressionMammalianMutationPromoterUGT8Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
FusionRed-Dectin1A-C-10
Plasmid#56111PurposeLocalization: Membrane, Excitation: 580, Emission: 608DepositorInsertDectin1A (CLEC7A Human)
UseTagsFusionRedExpressionMammalianMutationPromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
mRuby2-Calnexin-N-14
Plasmid#55891PurposeLocalization: Endoplasmic Reticulum, Excitation: 559, Emission: 600DepositorInsertCalnexin (CANX Human)
UseTagsmRuby2ExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceMarch 12, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS2+8 Flag2AUF1(p37)
Plasmid#135944PurposeExpresses AUF1p37 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.DepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
UseTagsFlag2ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G_NT-GSDMD_I105N_
Plasmid#201174PurposeDox-inducible expression of NT-GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D N-terminal domain (Gsdmd Mouse)
UseRetroviralTagsExpressionMutationaa1-276 only, I105NPromoterTRE3GAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223 LC3A no-stop
Plasmid#123204PurposeGateway entry clone encoding human MAP1LC3A lacking stop codon, suitable for C-terminal taggingDepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseGateway entry vector / entry cloneTagsExpressionMutationStop codon removedPromoterAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2021
Plasmid#49128PurposeAAV plasmid with DCX (Ple302) promoter driving expression of iCre.DepositorInsertssAAV-Ple302-iCre
UseAAVTagsExpressionMammalianMutationPromoterDCXAvailable sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_GNAI3_G-alpha
Plasmid#110014PurposeProtein expression and purification of GNAI3_G-alphaDepositorInsertGNAI3_G-alpha (GNAI3 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN_mMaroon-KIX-mMaroon
Plasmid#137009PurposeAAV backbone, mMaroon-KIX acceptor for R-CREBDepositorInsertKIX domain (Crebbp Rat)
UseAAVTagsmMaroonExpressionMammalianMutationPromoterhSynapsinAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEMS1977
Plasmid#49109PurposeAAV plasmid with C8ORF46 (Ple251) promoter driving expression of iCre.DepositorInsertssAAV-Ple251-icre
UseAAVTagsExpressionMammalianMutationPromoterC8ORF46Available sinceMay 10, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-mGSDMD1-251
Plasmid#111565PurposeExpress ELANE-cleaved mouse GSDMD in mammalian cellsDepositorInsertGasdermin D (Gsdmd Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV 3xFLAG-LC3A G120A
Plasmid#123090PurposeExpresses 3xFLAG-LC3A G120A in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
UseTags3xFLAGExpressionMammalianMutationGlycine 120 to AlaninePromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TFORF1028
Plasmid#143460PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertRARA (RARA Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEMS2157
Plasmid#70118PurposePromoterless AAV plasmid with MCS to insert promoter for expression of EmGFP. Contains WPRE.DepositorInsertssAAV-MCS-EmGFP-WPRE
UseAAVTagsExpressionMammalianMutationPromoterPromoterlessAvailable sinceMay 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSK+ mBMPR-1B DN
Plasmid#49530Purposecloning vector for dominant negative mouse BMP receptor 1BDepositorInsertBMPR-1B DN (Bmpr1b Mouse)
UseTagsExpressionBacterialMutationdominant negative mutation (K231R); also L2VPromoterT3Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD_NoCys-Myc
Plasmid#201169PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycExpressionMutationC39A, C57A, C77A, C122A, C265A, C192APromoterMESVAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only