1. p416 103Q GAL

   Plasmid

   #1186

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. BB3eH_AD

   Plasmid

   #98545

   Purpose

   empty BB3 for insertion and overexpression of 3 genes in P. pastoris

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BB1_12

   Plasmid

   #98495

   Purpose

   empty BB1 for promoter cloning

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. BB1_23

   Plasmid

   #98496

   Purpose

   empty BB1 for cds cloning

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. BB1_34

   Plasmid

   #98497

   Purpose

   empty BB1 for terminator cloning

   Depositor

   Diethard Mattanovich, Michael Sauer, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pILGFPB6

   Plasmid

   #83557

   Purpose

   Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL10 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMiR-HPV16-E7/E1-miR-375-m1-5

   Plasmid

   #53704

   Purpose

   Luciferase reporter assay for HPV16 E7/E1 with point mutation on miR-375 binding site

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   HPV16-E7/E1
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation in miR-375 binding sites m1-5 (refer to …

   Promoter

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMiR-HPV16-E1-m5

   Plasmid

   #53702

   Purpose

   Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   HPV16-E1(partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation in miR-375 binding site m5 (refer to cit…

   Promoter

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pMiR-HPV16-E1-m4

   Plasmid

   #53701

   Purpose

   Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   HPV16-E1(partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation in miR-375 binding site m4 (refer to cit…

   Promoter

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pMiR-HPV16-E1-m3

   Plasmid

   #53700

   Purpose

   Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site

   Depositor

   Edward Chan

   Article

   Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

   Insert

   HPV16-E1(partial)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Mutation in miR-375 binding site m3 (refer to cit…

   Promoter

   Available since

   June 26, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pMiR-HPV16-E7-m2

    Plasmid

    #53698

    Purpose

    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    HPV16-E7
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation in miR-375 binding site m2 (refer to cit…

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pMiR-HPV16-E7-m1

    Plasmid

    #53697

    Purpose

    Luciferase reporter assay for HPV16 E7 with point mutation on miR-375 binding site

    Depositor

    Edward Chan

    Article

    Jung et al Mol Cancer. 2014 Apr 8;13(1):80. doi: 10

    Insert

    HPV16-E7
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Mutation in miR-375 binding site m1 (refer to cit…

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

12. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS3Clux

    Plasmid

    #55172

    Purpose

    lux-reporter vector, integration at sacA, ampr, cmr

    Depositor

    Thorsten Mascher

    Article

    Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

    Type

    Empty backbone

    Use

    Synthetic Biology; Bacillus biobrick box

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CW3SL-EGFP

    Plasmid

    #61463

    Purpose

    AAV vector with CaMKIIa promoter, EGFP, and W3SL(shorten expression cassette with relatively high expression to maximize expression of larger genes)

    Depositor

    Bong-Kiun Kaang

    Article

    Choi et al Mol Brain. 2014 Mar 11;7:17. doi: 10.118

    Insert

    EGFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKIIa

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pR26 CAG AsiSI/MluI

    Plasmid

    #74286

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEF-XMAS-1xStop

    Plasmid

    #164411

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDT-sgRNA-XMAS-1x

    Plasmid

    #164413

    Purpose

    Dual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CMPE-ePPEplus

    Plasmid

    #205242

    Purpose

    For multiplex plant prime editing in wheat plants or monocotyledons protoplasts

    Depositor

    Zong Yuan

    Article

    Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

    Insert

    Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Detailed in manuscript

    Promoter

    Available since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pR26 CAG/BFP Dest

    Plasmid

    #74282

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter, loxP flanked stop cassette, BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-EF1a-i53

    Plasmid

    #216243

    Purpose

    Express i53 in mammalian cells as a HDR enhancer

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    i53
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge