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Plasmid#199768PurposeExpresses split-intein fused MSP1D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1D1
UseTagsHis6-TagExpressionBacterialMutationexternal His-Tags flanking the C and N terminiPromoterAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMO9075
Plasmid#117481Purposeempty complementation vector for Desulfovibrio: contains promoter from Aph(3')-IIa but no RBS, contains pBG1 and pMB1 replicons, SpecRDepositorInsertAph(3')-IIa promoter
UseTagsExpressionBacterialMutationPromoterAph(3')-IIa promoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psRaf1
Plasmid#89364PurposeA lenti-viral vector expressing photoswitchable Raf1 in mammalian cellsDepositorInsertpsRaf1 (RAF1 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFP
Plasmid#180475PurposeInducible gene expression vector pubb-QFDBD-2x AD*-VP16*-EcR, α-cry:EGFPDepositorInsertZebrafish ubb promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
NylCK_pET28a
Plasmid#215422PurposeExpression construct for NylCK gene, codon optimized for expression in E. coliDepositorInsertNylCK
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
human p53-(311-393)
Plasmid#24869DepositorInsertp53 (TP53 Human)
UseTagsGSTExpressionBacterialMutationContains aa 311-393.PromoterAvailable sinceAug. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorInsertsUseLentiviralTagsT2AExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NPHS-bio-His
Plasmid#53334PurposeExpresses full-length Nephrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertNPHS (NPHS1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-OSH2-2xPH
Plasmid#161987PurposeMammalian expression vector, contains two tandem repeats of OSH2 PH domain (PI(4)P biosensor). Fused to eGFPDepositorInsertOSH2 (OSH2 Budding Yeast)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
Intron-Tagging-mScarlet-Frame1-Parental-Minicircle
Plasmid#216125PurposeParental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.DepositorInsertsgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-ΔPS-PM,CS-RBSBBa_B0034-geneVI
Plasmid#134356PurposeAccessory plasmidDepositorInsertGene VI
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSC27
Plasmid#117478Purposemobilizable, SRB-stable, kanamycin-resistant cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
mGFP-hNET
Plasmid#193364Purposeexpression of human norpinephrine transporter tagged with monomeric GFP at the N-terminus in mammalian cellsDepositorInserthuman Norepinephrine Transporter (SLC6A2 Human)
UseTagsmonomeric GFP (mGFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TEX264-GFP
Plasmid#201925PurposeFor lentiviral transduction of TEX264-GFPDepositorInsertTEX264 (TEX264 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TEX264ΔLIR-GFP
Plasmid#201926PurposeFor lentiviral transduction of TEX264ΔLIR-GFPDepositorInsertTEX264 (TEX264 Human)
UseLentiviralTagsExpressionMutationF273APromoterAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIRIGF-IKZF3
Plasmid#69046Purposeexpression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.DepositorInsertIKZF3 (IKZF3 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterIRESAvailable sinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-NDH6
Plasmid#105503PurposeExpression of an N-terminally truncated form of Drosophila topoisomerase I with a C-terminal 6His tagDepositorInsertNDH6
UseTags6xHisExpressionMutationPromoterAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP220
Plasmid#185921PurposeBroad host range IncP promoter probe using lacZDepositorTypeEmpty backboneUseIncpTagsLacZExpressionBacterialMutationPromoterNoneAvailable sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAC103-PBNeo-U6-sgRNA-Expression
Plasmid#48228PurposesgRNA expression on PiggyBac vector with Neo-selectable markerDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ
Plasmid#178320PurposeMultiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.DepositorInsertsHRE RedFirefly reporter
P53 FLuc reporter
AP-1 Renilla reporter
FOXO NLuc Reporter
SMAD GrRenilla reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC2
Plasmid#172995Purposeconstitutive expression of FLAG-tagged WWC2DepositorInsert3xFLAG-WWC2 (WWC2 Human)
UseTags3xFLAGExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN3
Plasmid#180284PurposeSpacer cassette (TU2)DepositorInsertConL1 - Spacer - ConR2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR-LANP
Plasmid#16175DepositorInsertAcidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pDU1
Plasmid#169817PurposepSpCas9(BB)-2A-tCD19 (pDU1)DepositorInserttCD19
UseTagsfusion proteinExpressionMammalianMutationPromoterCbhAvailable sinceDec. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-ANP32A
Plasmid#31563DepositorInsertacidic (leucine-rich) nuclear phosphoprotein 32 family, member A (ANP32A Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP
Plasmid#134357PurposeReporter plasmidDepositorInsertsGFP
mCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7m-psMEK2
Plasmid#89363PurposeA lenti-viral vector expressing photoswitchable MEK2 in mammalian cellsDepositorInsertpsMEK2 (MAP2K2 Synthetic, Human)
UseLentiviralTagsHuman influenza hemagglutinin (HA) tag and pdDron…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shSmad4
Plasmid#37046DepositorInsertSmad4 (SMAD4 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only