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pGP-CMV-GCaMP3-44

Plasmid

#91780

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-44

Use

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCI-neo-myc-LC3(deltaC22)

Plasmid

#45448

Depositor

Tamotsu Yoshimori

Article

Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

Insert

microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)

Use

Tags

myc

Expression

Mammalian

Mutation

Encodes amino acids 1-120

Promoter

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKalpha FL WT

Plasmid

#33327

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40

Available since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-β-arrestin2 RRK/Q-5-kinase domain fusion protein (RRK5K)

Plasmid

#38262

Depositor

Robert Lefkowitz

Article

Nelson et al J Biol Chem. 2008 Jul 25;283(30):21093-1

Insert

β-arrestin2 (Arrb2 Rat)

Use

Tags

Flag and PIP5K Iα core kinase domain (residues 18…

Expression

Mammalian

Mutation

Arg 233, Arg237, and Lys251 converted to Glutamin…

Promoter

CMV

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
pDC146

Plasmid

#169366

Purpose

Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

Depositor

Axel Brunger

Article

Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

Insert

Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

Use

Tags

6 his tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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mEmerald-CathespinB-6

Plasmid

#54024

Purpose

Localization: Lysosomes, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

CathespinB

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
KIF5C(1-560)-GFP11

Plasmid

#61670

Purpose

Non-fluorescent, truncated kinesin-1 fused to split GFP linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-GFP11 (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-mCitrine

Plasmid

#61676

Purpose

Single mCitrine-labeled, truncated kinesin-1

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KHC(1-560)-mCitrine (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PS-CFP2-Cx26-7

Plasmid

#57222

Purpose

Localization: Gap Junctions, Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

Cx26 (Gjb2 Rat)

Use

Tags

PS-CFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMA3379

Plasmid

#46874

Purpose

A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

Depositor

Troy Stevens, Mikhail Alexeyev

Article

Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

Insert

soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)

Use

Lentiviral

Tags

Myc

Expression

Mutation

Promoter

TRE-Tight

Available since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform IV

Plasmid

#120320

Purpose

Septin 11 isoform IV mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV0-OCT-HA-eGFP

Plasmid

#67479

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
Septin 11 isoform I

Plasmid

#120314

Purpose

Septin 11 isoform I mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Cx26-7

Plasmid

#55021

Purpose

Localization: Gap Junctions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Cx26

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
gamma1-EGFP

Plasmid

#119224

Purpose

GABAA receptor expression (gamma 1 subunit with EGFP tag at the C-terminus)

Depositor

Angel de Blas

Insert

GABRG1 (Gabrg1 Rat)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFLAG rat Antizyme 1

Plasmid

#11183

Depositor

Bruce Zetter

Article

Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

Insert

AZ1 (Oaz1 Rat)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 17, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
PA-mCherry-miniSOG-Cx43-7

Plasmid

#57787

Purpose

Localization: Gap Junctions, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Cx43

Use

Tags

PA-mCherry-miniSOG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
mEos2-CathespinB-6

Plasmid

#57354

Purpose

Localization: Lysosomes, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

CathespinB (Ctsb Rat)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLJM60-S6K1(T389S) barcoded

Plasmid

#48800

Purpose

expresses mutant S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 T389S (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGTACC

Expression

Mammalian

Mutation

T389S

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only