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Showing: 1241 - 1260 of 49625 results

  1. HA-Clover-HIF-1alpha Wildtype

    Plasmid
    #163365
    Purpose
    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163365-plasmid-map-sequence-id-322246

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  2. pJAT32

    Plasmid
    #204297
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204297-plasmid-map-sequence-id-405508

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  3. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

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  4. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid
    #217302
    Purpose
    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L
    Depositor
    Beverly L. Davidson
    Insert
    XS3B-miniXon
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217302-plasmid-map-sequence-id-436485

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  5. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

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  7. pJAT77

    Plasmid
    #204295
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204295-plasmid-map-sequence-id-406178

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  10. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid
    #159908
    Purpose
    Mutagenesis of Htr2a
    Depositor
    Larry Zweifel
    Insert
    Htr2a (Htr2a Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159908-plasmid-map-sequence-id-317314

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  11. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Has Service
    Lentiviral Prep
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  12. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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  16. XLone-Puro SARS-CoV2 N P2A eGFP-NLS

    Plasmid
    #154399
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and nuclear eGFP
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154399-plasmid-map-sequence-id-304590

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  17. CHD1 (CHD1A-c107)

    Plasmid
    #110253
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CHD1 (CHD1 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110253-plasmid-map-sequence-id-212335

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  18. ABCB8 (ABCB8A-c016)

    Plasmid
    #110266
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    ABCB8 (ABCB8 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110266-plasmid-map-sequence-id-212276

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  19. pARNTL.1.0-gDNA

    Plasmid
    #112480
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ARNTL
    Depositor
    Kevin White
    Insert
    ARNTL (BMAL1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112480-plasmid-map-sequence-id-230516

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  20. AAV-SYN-PIK3CD

    Plasmid
    #203730
    Purpose
    AAV vector plasmid expressing human phosphoinositide 3-kinase catalytic subunit delta (PIK3CD) under the human synapsin (SYN) promoter
    Depositor
    Bart Nieuwenhuis, Richard Eva
    Insert
    PIK3CD (PIK3CD Human)
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human synapsin promoter
    Available since
    July 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    203730-plasmid-map-sequence-id-402632

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Showing: 1241 - 1260 of 49625 results