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pSpCas9_BB_2A-GFP_MAPRE3-gRNA#3

Plasmid

#107731

Purpose

Knock out of EB3 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9_BB_2A-GFP_MAPRE1-gRNA#3

Plasmid

#107728

Purpose

Knock out of EB1 in human cells by CRISPR/Cas9

Depositor

Torsten Wittmann

Article

van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

Insert

MAPRE1 gRNA #3 (targets Exon 1) (MAPRE1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-hCD4-GCaMP2

Plasmid

#18932

Depositor

Karel Svoboda

Article

Mao et al PLoS ONE. 2008 . 3(3):e1796.

Insert

hCD4-GCaMP2 (CD4 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2008

Availability

Academic Institutions and Nonprofits only
pCAG/hArf4(WT)-EGFP

Plasmid

#79407

Purpose

Expresses C-terminally EGFP-tagged Arf4(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Nakai et al Mol Biol Cell. 2013 Aug;24(16):2570-81.

Insert

Arf4 (ARF4 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4 1-747

Plasmid

#71399

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-747 (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-SRGAP3FBAR-EGFP

Plasmid

#168506

Purpose

Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3-FBAR (SRGAP3 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-492

Promoter

CAG

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pACYC-OspCas12c-noncoding

Plasmid

#120878

Purpose

Expresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.

Depositor

Arbor Biotechnologies

Article

Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

Insert

Noncoding sequences for CRISPR-OspCas12

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

lac

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pX330_CALR sgRNA / hSpCas9

Plasmid

#172838

Purpose

Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pBT225_(pCA-tdT3Myc)

Plasmid

#36873

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tdTomato-3Myc

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCAG-AmCas12b-2AeGFP

Plasmid

#121947

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AmCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Intron4-PAFAH1B1

Plasmid

#92434

Purpose

expresses Cas9 and gRNA targeting PAFAH1B1

Depositor

Matthias Leisegang, Ralf Brandes

Article

Josipovic et al Acta Physiol (Oxf). 2016 Apr 28. doi: 10

Insert

PAFAH1B1 (PAFAH1B1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-RNF169UBD

Plasmid

#119011

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCA24N-ligase-pprA

Plasmid

#87744

Purpose

IPTG-inducible expression of ligase-pprA fusion for protein purification

Depositor

Wayne Patrick

Article

Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

Insert

T4 DNA ligase (30 )

Use

Tags

6xHis and codon optimized pprA (from Deinococcus …

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCALNL-BsaI-EGFP

Plasmid

#123136

Purpose

Golden Gate acceptor vector for the assembly of EGFP-based mammalian recombinase reporters.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

EGFP, mRFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

cBA

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-LexAQFw

Plasmid

#61311

Purpose

Expresses chimeric transactivator LexA_DBD:QF2w_AD in Drosophila

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA_DBD::QF2w_AD

Use

Tags

Expression

Insect

Mutation

Changed EK on C-terminus of QF2 to KKKK

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-eSpCas9

Plasmid

#126769

Purpose

Expression of increased fidelity eSpCas9 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

eSpCas9

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG aconitase GFP

Plasmid

#66104

Purpose

expression of chicken aconitase GFP

Depositor

Martin Scaal, Felicitas Proels

Article

et al Development. 2015 Feb 15;142(4):665-71.

Insert

aconitase

Use

Chicken expression

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only
pCaSpeR3/ncd-gfp #10

Plasmid

#66082

Purpose

stable germline transformation of Drosophila to produce Ncd-GFP expression

Depositor

Sharyn Endow

Article

Endow et al J Cell Biol. 1997 Jun 16;137(6):1321-36.

Insert

Ncd kinesin-14 motor protein (ncd Fly)

Use

Tags

GFP

Expression

Insect

Mutation

D699L

Promoter

ncd promoter (3.1kb upstream region of ncd start …

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
pCAG-HDAC4 1-129

Plasmid

#71401

Purpose

CAG promoter, mammalian expression

Depositor

Bo Chen

Article

Guo et al Nat Commun. 2015 Aug 14;6:8005. doi: 10.

Insert

HDAC4 1-129 (HDAC4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only
pCAGGS-TAL-NC2

Plasmid

#43856

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

TALEN-FokI nuclease backbone

Use

Tags

Flag and FokI nuclease

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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