Addgene

We narrowed to 4,957 results for: hwa

Showing: 1241 - 1260 of 4957 results

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Δ741-752-GFP

Plasmid

#33119

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of aa 741-752

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pS2-FLAG-Sestrin2-E451A

Plasmid

#73679

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

Sesn2

Available since

April 12, 2016

Availability

Academic Institutions and Nonprofits only
Wnt5B STOP

Plasmid

#35875

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5B (WNT5B Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pBAD18HMGR

Plasmid

#19041

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

truncated 3-hydroxy-3-methyl-glutaryl-coenzyme A Reductase (HMG1 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pcDNA-Wnt8B-V5

Plasmid

#35936

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSG5-p85α-ΔiSH2-N

Plasmid

#39498

Depositor

Julian Downward

Article

Rodriguez-Viciana et al Cell. 1997 May 2;89(3):457-67.

Insert

p85 ΔiSH2-N (PIK3R1 Bovine)

Use

Eukaryotic

Tags

Expression

Mutation

ΔiSH2-N = deletion of AA478-513

Promoter

Available since

Aug. 30, 2012

Availability

Academic Institutions and Nonprofits only
Hy_pMT dati MCS circfirefly_1677

Plasmid

#110118

Purpose

Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE

Plasmid

#101116

Purpose

Encodes dCas9(N) fused to LPAR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

LPAR1-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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γA3/α1-GFP

Plasmid

#33112

Depositor

Greg Phillips

Article

O'Leary et al Mol Biol Cell. 2011 Sep 14.

Insert

Pcdh-γa3 (Pcdhga3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Cytoplaspic domain has been replaced with the cyt…

Promoter

CMV

Available since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pDGE65

Plasmid

#79447

Purpose

recipient plasmid [single nuclease]; pPcUbi:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE146

Plasmid

#79449

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE167

Plasmid

#79457

Purpose

recipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDGE168

Plasmid

#79458

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, Hygro

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-spo5DSR

Plasmid

#49867

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Expression

Mutation

Promoter

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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slpr genomic fragment

Plasmid

#42074

Depositor

Norbert Perrimon

Article

Stronach et al Genes Dev. 2002 Feb 1;16(3):377-87.

Insert

slpr genomic fragment (slpr Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Hsp70

Available since

March 6, 2013

Availability

Academic Institutions and Nonprofits only
Wnt7A Non-STOP

Plasmid

#35896

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt6 STOP

Plasmid

#35876

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt6 (WNT6 Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation G (567) --> A

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt8B STOP

Plasmid

#35880

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt8B (WNT8B Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
Wnt16 STOP

Plasmid

#35886

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt16 (WNT16 Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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M27 NacMS>luc

Plasmid

#17177

Depositor

Randall Moon

Article

Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

Insert

nacre promoter (mitfa Zebrafish)

Use

Luciferase

Tags

luciferase

Expression

Mutation

All three Tcf/Lef sites are mutated

Promoter

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only