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pRK5-mEGFP-HMGB3-MUT

Plasmid

#194565

Purpose

Mammalian Expression of mEGFP-HMGB3 Mutant (K161Ifs*55) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

K161Ifs*55

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-HMGB1-delFS

Plasmid

#194555

Purpose

Mammalian Expression of mEGFP-HMGB1 WT Fusion Protein, "del FS" variant

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

truncation by stop codon after Lys183

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-DVL1-MUT

Plasmid

#194587

Purpose

Mammalian Expression of mEGFP-DVL1 Mutant (H502Pfs*143) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

DVL1 (DVL1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

H502Pfs*143

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-CALR-MUT

Plasmid

#194579

Purpose

Mammalian Expression of mEGFP-CALR Mutant (D387Rfs*44) Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

CALR (CALR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

D387Rfs*44

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pFH75

Plasmid

#128224

Purpose

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

classic sgRNA backbone for tRNA-gRNA array, Position 1, combine with TaU6 promoter module (pICSL90003)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Use

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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FlucWT-HA-GFP11-N1

Plasmid

#91954

Purpose

Expresses FlucWT-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucWT-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.336

Plasmid

#108528

Purpose

LacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LacIAM + PTac-YFP

Use

Tags

Expression

Bacterial

Mutation

LacI Q18M A47V F161Y

Promoter

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pJG103

Plasmid

#158533

Purpose

wrmScarlet11 x3 tandem repeats

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet11 x3 tandem repeats

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAJM.657

Plasmid

#108525

Purpose

CymRAM + PCymRC-YFP Reporter. Cuma sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

CymRAM + PCymRC-YFP

Use

Tags

Expression

Bacterial

Mutation

CymR: S110G A171V

Promoter

Available since

Aug. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.771

Plasmid

#108534

Purpose

NahRAM +PSalTTC-YFP Reporter. Sal sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

NahRAM + PSalTTC-YFP

Use

Tags

Expression

Bacterial

Mutation

NahR: I25V P72H V118A R248C

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.690

Plasmid

#108533

Purpose

PcaUAM + P3B5B-YFP Reporter. DHBA sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PcaUAM + P3B5B-YFP

Use

Tags

Expression

Bacterial

Mutation

PcaU: K14N A165T N201H

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.474

Plasmid

#108526

Purpose

LuxR + PLuxB-YFP Reporter. OC6 sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

LuxR + PLuxB-YFP

Use

Tags

Expression

Bacterial

Mutation

VanR: T49I A111V P179S

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.661

Plasmid

#108532

Purpose

TtgRAM + PTtg-YFP Reporter. Nar sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

TtgRAM + PTtg-YFP

Use

Tags

Expression

Bacterial

Mutation

TtgR: R130H A144T A148V

Promoter

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.677

Plasmid

#108530

Purpose

AraCAM + AraE + PBAD-YFP Reporter. Ara sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AraCAM + AraE + PBAD-YFP

Use

Tags

Expression

Bacterial

Mutation

AraC: L133F E165L E169G C280L

Promoter

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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GST-HA-GFP11-N1

Plasmid

#91953

Purpose

Expresses GST-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

GST-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAJM.884

Plasmid

#108536

Purpose

AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AcuRAM + PAcu-YFP

Use

Tags

Expression

Bacterial

Mutation

AcuR: E80K R154H L202M

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.683

Plasmid

#108531

Purpose

BetIAM + PBetI-YFP Reporter. Cho sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

BetIAM + PBetI-YFP

Use

Tags

Expression

Bacterial

Mutation

BetI: S115I

Promoter

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.969

Plasmid

#108537

Purpose

MphRAM + EryR + PMph-YFP Reporter. Ery sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

MphRAM + EryR + PMph-YFP

Use

Tags

Expression

Bacterial

Mutation

MphR: N94D

Promoter

Available since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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FlucSM-HA-GFP11-N1

Plasmid

#91955

Purpose

Expresses aggregation-prone FlucSM-HA-GFP11 in mammalian cells

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

FlucSM-HA-GFP11

Use

Tags

Expression

Mammalian

Mutation

R188Q

Promoter

CMV

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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