We narrowed to 31,661 results for: uros
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Plasmid#107129PurposeInducible expression of Flag-tagged SMAD4 in mammalian cellsDepositorInsertSMAD4
UseLentiviralTagsFLAGAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMK364 (CMV-OsTIR1-loxP-PURO-loxP)
Plasmid#121184PurposeOsTIR1 introductionDepositorInsertCMV-OsTIR1-loxP-PURO-loxP
UseCRISPRMutationOsTIR1 codon optimized for human cellsAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-IRES-Puro GFP-3xFLAG
Plasmid#201636PurposeOver-expression of 3xFLAG-tagged GFPDepositorInsertGFP
UseLentiviralTags3xFLAGAvailable SinceJuly 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin
Plasmid#134661PurposeExpresses EGFP-tagged synaptic vesicle proteinDepositorInsertvesicle-associated membrane protein 2 (VAMP2 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG J591-scFV T-CAR
Plasmid#171961PurposeDonor plasmid for J591 T-CAR expression in human cellsDepositorInsertSynthetic J591-scFV CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}
Plasmid#232879PurposeExpresses the 5-HT6R coupled to the RGECO1 calcium sensorDepositorInsert5-Hydroxytryptamine Receptor 6 (HTR6 Human)
UseLentiviralTagsRGECO1.1ExpressionMammalianPromoterCMVAvailable SinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-ApaLI*-FLAG-T2A-mTagBFP2-Puro
Plasmid#211463PurposeLentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI mutant (D211A, E225A and K227A)DepositorInsertApaLI-dead
UseLentiviralTagsFLAGExpressionMammalianMutationD211A, E225A, K227APromoterTREAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-SNAP-TERT
Plasmid#71390PurposeHomologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.DepositorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hU6-enhanced_gRNA-BFP-P2A-PuroR
Plasmid#230935PurposegRNA cloning vector encoding BFP and puromycin resistanceDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsTagBFPPromoterhU6Available SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-VAMP5-IRES-Puromycin
Plasmid#134662PurposeExpresses EGFP-tagged plasma membrane markerDepositorInsertvesicle-associated membrane protein 5 (VAMP5 Human)
UseLentiviralTagsEGFPExpressionMammalianPromoterEF1aAvailable SinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a
Plasmid#177435PurposeTo express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.DepositorInsertPhosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
UseLentiviralTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre-P2A-tdTomato-T2A-Puro
Plasmid#72255PurposeExpress iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoterDepositorInsertiCre-P2A-tdTomato-T2A-Puro
UseLentiviralExpressionMammalianPromoterCB and chicken beta-actin + CMV enhancerAvailable SinceMarch 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
Plasmid#135814PurposeFluorescent reporter for knock-in of TdTomato into the TH locusDepositorInsertTH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression. (TH Human)
Tagsp2a-TdTomatoExpressionMammalianAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gCD44v2)-EF1a-BFP-Puro-Cas9(FZ)
Plasmid#117134PurposeAll-in-one expression vector for Cas9 and gRNA against CD44DepositorInsertgCD44 v2 (Cd44 Synthetic)
UseCRISPR and LentiviralMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, human EF1a promoterAvailable SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-MSCV-hCD38-EF1α-GFP+Puro
Plasmid#134936Purposeexpression human CD38 in mammalian cellsDepositorAvailable SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240107PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
ExpressionMammalianAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB-CMV-MCS-Puro dr_cPLA2-mKate2
Plasmid#163844PurposeUsed as a driver for the readout of cPLA2 activation in live cells with an mKate2 tag.DepositorInsertcytosolic phospholipase a2 (pla2g4aa Zebrafish)
TagsmKate2ExpressionMammalianPromoterCMVAvailable SinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-EF1A-mNeonGreen2x-SREBP1-P2A-Puro
Plasmid#228401PurposeEncodes a N-terminal truncated version of SREBP1 lacking part of the transcriptional activation domain and fused to a tandem of mNeongreen to visualize the import of SREBP1DepositorInsertSREBP1 (SREBF1 Human)
TagsmNeonGreen2xExpressionMammalianMutationdeleted N terminal activation domain (amino acids…PromoterEF1aAvailable SinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen-NLS. PGK-puro
Plasmid#136262PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreen-NLSExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT K842M (Puro)
Plasmid#154291PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, K842M variant, catalytic dead)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationK842M mutation (Catalytic Inactive)PromoterPGKAvailable SinceDec. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG PSMA nanobody T-CAR
Plasmid#171962PurposeDonor plasmid for PSMA nanobody T-CAR expression in human cellsDepositorInsertSynthetic anti-PSMA nanobody CAR
UseCRISPR, Synthetic Biology, and TALENExpressionMammalianPromoterCAGAvailable SinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti spCas9 T2A iRFP670 P2A puro
Plasmid#122182PurposeThe plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.DepositorAvailable SinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro METTL3
Plasmid#200724PurposeLentiviral expression of Flag-Tagged human METTL3DepositorInsertMETTL3 (METTL3 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
lenti-CMV-TFAM-BioID2-IRES-Puro
Plasmid#211465PurposeLentivirus backbone expressing human TFAM fused with BioID2DepositorInsertTFAM-BioID2
UseLentiviralTagsFLAGExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSCV-EZH2-PGK-Puro-IRES-GFP
Plasmid#75125PurposeRetroviral expression plasmid encoding wild type human EZH2DepositorAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro GFP-p62 K7A, D69A
Plasmid#38281DepositorInsertSequestosome-1 (Sqstm1 Mouse)
UseRetroviralTagsEGFPExpressionMammalianMutationchanged K7A, D69AAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorInsertRenilla
UseLentiviralExpressionMammalianAvailable SinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-FLIP-puro-dsRed-GFP-miRNA
Plasmid#32704DepositorInsertemGFP-miRNA
UseCre/Lox, RNAi, and RetroviralExpressionMammalianAvailable SinceDec. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
N114 nLV EF-1a-Gal4-VP16-PGK-Puro
Plasmid#44014DepositorInsertGal4/DBD (1-147)
UseLentiviralTagsVP16/Activation/DomainPromoterEF-1aAvailable SinceJune 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-wpA-pPuroTK
Plasmid#214127PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-VPR
UseCRISPRExpressionMammalianMutationCodon-optimized for mammalian cellsAvailable SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-mTagBFP2-2A-Puro_hU6-DJ-DR36-BsmBI
Plasmid#226009PurposeDjCas13d guide RNA cloning backbone (lenti)DepositorTypeEmpty backboneUseLentiviralAvailable SinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro LARP7
Plasmid#200725PurposeLentiviral expression of Flag-Tagged human LARP7DepositorInsertLARP7 (LARP7 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianPromoterCMVAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT1-2A-eGFP PGK PuroR Donor Plasmid
Plasmid#82333PurposeWT1 targeting donorDepositorUseCRISPR and TALENTags2A-eGFPExpressionMammalianAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro-GFP-BamHI-IRES-mCherry
Plasmid#232247PurposeAcGFP1-BamHI-IRES-mCherry empty backboneDepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-pSV40(1)-pmRevErbA-mScaI3-NLS-Pest
Plasmid#240116PurposeA lentiviral (red) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by SV40 promoter fragment.DepositorInsertmurine Nr1d1 promoter+mScarlet-I3-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded SV40-EnhancerAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pL6puro-JeT-pmRevErbA-Electra2-NLS-Pest
Plasmid#240111PurposeA lentiviral (blue) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.DepositorInsertmurine Nr1d1 promoter+Electra2-NLS-Pest-Reporter (Nr1d1 Mouse)
UseLentiviralMutationadded JeT PromoterAvailable SinceAug. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK Puro GFP-LC3B G120
Plasmid#123241PurposeExpression vector with PGK promoter for low expression of EGFP-LC3B G120. For lentivirus production and stable transduction in mammalian cells.DepositorInsertMicrotubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
UseLentiviralTagsEGFPExpressionMammalianMutationDeleted amino acids 121-125. Stop codon after G12…PromoterPGKAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2
Plasmid#202762PurposeEncodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harborDepositorAvailable SinceFeb. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro
Plasmid#120561PurposeExpresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus productionDepositorAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW-HA-hRb-WT-T373A-S608A-S612A-puro
Plasmid#212674PurposeExpress tagged hRb mutantDepositorAvailable SinceAug. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor
Plasmid#86843PurposeHR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.DepositorInsertFLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
TagsFLAG-HaloTagExpressionMammalianPromoterEndogenous TERT promoterAvailable SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-BICRAL-FLAG-pgk-puro
Plasmid#107732PurposeTet inducible lentiviral C-terminal FLAG BICRAL (GLTSCR1L) ORFDepositorAvailable SinceMarch 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS
Plasmid#127656PurposeOver-express NLS-FLAG-cGASDepositorAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR
Plasmid#162334PurposeLentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistanceDepositorInsertdSaCas9-KRAB
UseLentiviralTagsHAExpressionMammalianPromoterhUbCAvailable SinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-K560-E236A-24xGCN4_v1-IRES-Puro
Plasmid#60909PurposeA minimal K560 kinesin rigor motor tagged with the SunTag for single molecule imagingDepositorInsertK560-E236A
ExpressionMammalianAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
HA-HIF2α P405A Δ450-572-pBabe-Puro
Plasmid#25958DepositorInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
UseRetroviralTagsHA-tagExpressionMammalianMutationcDNA changed amino acids: Proline 405 to Alanin…Available SinceSept. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
pLenti-C-Myc-DDK-IRES-Puro HNRNPA1
Plasmid#200727PurposeLentiviral expression of Flag-Tagged human HNRNPA1DepositorInsertHNRNPA1 (HNRNPA1 Human)
UseLentiviralTagsMyc-DDK from backbone plasmidExpressionMammalianPromoterCMVAvailable SinceJuly 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2-hUbC-dCas9-KRAB-T2a-Puro
Plasmid#225999PurposeTo establish stable dCas9-KRAB knock-in lineDepositorInserthumanized dCas9-KRAB T2A Puro
UseSleeping beauty transposonExpressionMammalianMutationD10A and H840AAvailable SinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only