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Plasmid#17477Purpose3rd gen lentiviral Luciferase expression vector, CMV promoter, PuroDepositorInsertFirefly Luciferase
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSIJ8
Plasmid#68122PurposeTemperature sensitive plasmid for either lambda Red recombinase genes or flippase recombinase expressionDepositorInsertsflp recombinase
rhaS
rhaR
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceDec. 8, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
SIRT3 Flag
Plasmid#13814PurposeMammalian expression of human SIRT3 with flag tagDepositorInsertSIRT3 (SIRT3 Human)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
circRNA-synIRES-R25-mNeonGreen
Plasmid#188115PurposeTemplate plasmid to transcribe circular RNA encoding mNeonGreenDepositorInsertmNeonGreen
UseTagsExpressionMammalianMutationPromoterT7Available sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD120
Plasmid#119879PurposeConstitutive expression of Che9c60 and Che9c61 recombinases on pB264/pBR322 backbone with kanamycin resistance.DepositorInsertpConstitutive_Che9c60 and Che9c61
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 phiC31
Plasmid#68310PurposeIn vitro transcription vector to generate phiC31 mRNA (i.e. for zebrafish injection).DepositorInsertphiC31
UseRna transcriptionTagsExpressionMutationPromoterCMVAvailable sinceAug. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyso-RA
Plasmid#209875PurposeDimerization dependent fluorescent protein RA anchored to cytosolic face of the lysosomal membrane with human LAMP1DepositorInsertddRFP A
UseTagsLAMP1ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNP1723
Plasmid#222727PurposesfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)DepositorTypeEmpty backboneUseTagssfGFPExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
Plenti-CMV-MCS-RFP-SV-puro
Plasmid#109377PurposeThis plasmid expresses dsRed2 and puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsdsRed2ExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDonor14-N7HELIX-KanR
Plasmid#190663PurposeKanamycin resistance donor for N7HELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequenceDepositorInsertKanR, R6K origin of replication
UseTagsExpressionBacterialMutationPromoterNAAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pConRef-2H12.D11
Plasmid#221151PurposecdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)DepositorInsertscdGreen2
mScarlet-I
UseTagsExpressionBacterialMutationN-terminus changed: starts with MSKKYGEAVIKE ...PromoterConstitutive and None (same as cdGreen2)Available sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-dCas9-KRAB-blast
Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABExpressionMutationD10A and H840APromoterAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV rtTA3 Blast (w756-1)
Plasmid#26429Purpose3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, BlastDepositorInsertTetracycline repressor A3 mutant
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
hLRP1-pEZT-BM
Plasmid#177985PurposeExpression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cellsDepositorInsertLDL receptor related protein 1 (LRP1 Human)
UseTagsNoExpressionMammalianMutationPromoterCMVAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL192 (TRV1)
Plasmid#148968PurposeTobacco Rattle Virus RNA 1; used for virus induced gene silencing in plants along with pYL156 (TRV RNA2)DepositorInsertTRV RNA1
UsePlant vector with trv rna1; this vector used with…TagsExpressionMutationP697S mutation in putative replicase. S239G and S…PromoterAvailable sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTNS2
Plasmid#64968PurposeTn7 transposase expressionDepositorInserttnsABCD
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
CYBL-SPI1-CEBPB-IRF5
Plasmid#190719PurposeFor microglia differentiation from iPSCDepositorInsertSPI1-CEBPB-IRF5
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#112924PurposepiggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant markerDepositorInsertEF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
UsePiggybac transposon vectorTagsExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT4-CMV-GFP
Plasmid#117046PurposeOptimized Sleeping Beauty Transposon Vector for GFP expression by a CMV promoterDepositorInsertGFP
UseTransposonTagsExpressionMutationPromoterCMVAvailable sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-mitoDsRed
Plasmid#44386Purpose3rd generation lentiviral vectorDepositorInsertmitoDsRed
UseLentiviralTags61 aa targeting sequence of the P1 isoform F1F0-A…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRFP-Lact-C2
Plasmid#74061PurposeFluorescent biosensor for phosphatidylserine.DepositorInsertLactadherin-C2 (MFGE8 Bovine)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-T7RNAP
Plasmid#124138PurposeBacterial expression plasmid for PURE system componentsDepositorInsertT7 RNA polymerase (T7p07 Enterobacteria phage T7)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Bxb1
Plasmid#182142PurposeExpression of human codon optimized Bxb1DepositorInserthuman codon optimized Bxb1
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-hM3D(Gq)-mCherry
Plasmid#44361PurposeDouble floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.DepositorHas ServiceAAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry
UseAAV; Adeno associated viral vectorTagsmCherryExpressionMutationPromoterhuman Synapsin 1Available sinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-GFP
Plasmid#37825DepositorHas ServiceAAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1InsertGFP
UseAAV; Adeno-associated virusTagsExpressionMammalianMutationN/APromoterCAGAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pVSV eGFP dG
Plasmid#31842DepositorInsertVSV genome
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_Bless
Plasmid#207255PurposeMammalian expression of Baboon Endogenous Retrovirus env proteinDepositorInsertBaboonEndogenousRetrovirus_Rless_env
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBeloBac11loxP2272
Plasmid#60342PurposeThis is a BAC vector that contains both wt and mutant loxP sites.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionBacterialMutationPromoterAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO-ChRmine-mScarlet-Kv2.1-WPRE
Plasmid#130999PurposeDouble floxed soma-targeted ChRmine-mScarlet under the control of Ef1a promoterDepositorInsertChRmine
UseAAVTagsmScarlet-Kv2.1ExpressionMutationPromoterEf1aAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
TetO-FUW-OSKM
Plasmid#20321PurposeLentiviral plasmid for tet-inducible expression of mouse Oct4, Sox2, Klf4 and Myc for iPS cell generationDepositorUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAVS1 T2 CRIPR in pX330
Plasmid#72833PurposeCRISPR/Cas to target the AAVS1 locus in human cellsDepositorInsertCas9 and gRNA for targeting the AAVS1 locus in human cells
UseCRISPRTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas9-sgAAVS1-2
Plasmid#129727PurposeExpressing SpCas9 and sgAAVS1-2DepositorInsertSpCas9 and sgAAVS1-2
UseCRISPRTagsT2A-mCherry-P2A-PuroExpressionMammalianMutationPromoterhU6Available sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-TO-NFIA.1-SOX9 (bi-directional promoter)
Plasmid#182306PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expressionUsePiggybacTagsNone and T2A-mycNLS-mTagBFP2ExpressionMammalianMutationPromoterTRE3G-Bi-directionalAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-MAFB-IRF8-CEBPA
Plasmid#190720PurposeFor microglia differentiation from iPSCDepositorInsertMAFB-IRF8-CEBPA
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#167547PurposeUsed for CRISPR-Cas9 mediated recomineering in Enterococcus. Can clone desired gRNA using BsaI digestion.DepositorInsertschloramphenicol acetyl transferase
pUC19 origin of replication
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NLS-HA-Bxb1
Plasmid#51271PurposeComponent of the Bxb1-rcCre binary site-specific recombinase system, expression of the site-specific recombinase Bxb1 (mouse codon-optimized)DepositorInsertNLS-HA-Bxb1
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-flag-GFP-iCAM-1
Plasmid#205186PurposeThis vector encodes of a synCAM (iCAM1) and GFPDepositorInsertsynCAM iCAM1 and GFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only