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Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
UseTagsMitochondrial targeting signalExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCAR_sgRNA_hygro-tagBFP-lox2272
Plasmid#162078Purpose3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistanceDepositorTypeEmpty backboneUseCRISPR, Cre/Lox, and LentiviralTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-NT*-HRV3CP
Plasmid#162795PurposeExpressess an optimized construct of human rhinovirus 14 3C protease (NT*-HRV3CP)DepositorInsertHuman rhinovirus 14 3C protease
UseTagsNT* solubility tagExpressionBacterialMutationPromoterT7Available sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
TAp63alpha-FLAG
Plasmid#27008DepositorInsertTAp63alpha (TP63 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-mCherry
Plasmid#50462PurposeDouble floxed mCherry under the control of EF1a promoterDepositorInsertmCherry
UseAAVTagsN/AExpressionMutationPromoterEF1aAvailable sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
UseTagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA
Plasmid#154868PurposeHuman synapsin-1 promoter; Flp-dependent hM3D(Gq) DREADD-mCherryDepositorHas ServiceAAV Retrograde and AAV8InserthM3D(Gq)-mCherry
UseAAV; Flp-frtTagsmCherryExpressionMammalianMutationPromoterHuman Synapsin-1Available sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-FGF2-G3
Plasmid#135521PurposeEngineered form of fibroblast growth factor 2 with improved thermostabilityDepositorInsertfibroblast growth factor 2 (FGF2 Human)
UseTags6x His tag and Agg Thrombin TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 PromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual wt p110α/p85α
Plasmid#199302Purposeco-expression of His-tagged p110α wt and Avi-tagged p85α wt proteinsDepositorUseTagsAvi and HisExpressionInsectMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMP71-HLA-A0201-His
Plasmid#108214PurposeHLA-A0201 his taggedDepositorInsertmajor histocompatibility complex, class I, B (HLA-A Human)
UseRetroviralTagshisExpressionMammalianMutationPromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpCas9-P2A-EGFP (RTW3027)
Plasmid#139987PurposeCMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationPromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCasKP-apr
Plasmid#117231PurposeBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella PneumoniaeDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27
Plasmid#163617Purposemouse SNX27 cDNA N-terminally tagged with GFPDepositorInsertmouse SNX27 (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmtb-t7-alpha-bungarotoxin
Plasmid#69542PurposeFor in vitro transcription of alpha-bungarotoxin mRNA that can be injected into embryos.DepositorInsertalpha-bungarotoxin
UseTol2, beta-actin promoter, sp6,t7 for zebrafishTagsExpressionMutationPromoterbeta-actin, sp6, t7Available sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
Twinkle-APEX2-V5
Plasmid#129705PurposeAPEX2 fused to the mitochondrial nucleoid protein TwinkleDepositorUseLentiviralTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pICE-RNaseHI-WT-NLS-mCherry
Plasmid#60365PurposePlasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE17
Plasmid#90359PurposeNFAT - PKC/Ca++ gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterNFATAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmU6-gRNA
Plasmid#53187PurposeExpresses the S. pyogenes sgRNA from the mouse U6 promoterDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromotermouse U6Available sinceJune 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-GRAB_rDA-mut
Plasmid#140558PurposeExpresses the red fluorescent DA-insensitive control sensor GRAB_rDA-mut in neuronsDepositorHas ServiceAAV9InsertRed fluorescent DA-insensitive sensor GRAB_rDA-mut (control sensor)
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only