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pPRIME-CMV-GFP-shDyn2

Plasmid

#132712

Purpose

Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(2) (Pdyn Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-NEW-fRGECO2

Plasmid

#125248

Purpose

Expresses red fluorescent calcium sensor in mammalian cells

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO2

Use

Tags

NES signal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGFP-Kif5cmut

Plasmid

#71854

Purpose

mammalian expression of mutant GFP-Kif5c

Depositor

Michelle Peckham

Article

Dunn et al J Cell Sci. 2008 Apr 1;121(Pt 7):1085-95

Insert

Kif5c (Kif5c Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T93N mutation and also a novel SpeI site (1652). …

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only
Ad-HisFlag-USP19CYT

Plasmid

#155245

Purpose

for generating adenoviruses expressing the cytoplasmic isoform of rat USP19

Depositor

Simon Wing

Article

Wiles et al Mol Biol Cell. 2015 Mar 1;26(5):913-23.

Insert

USP19 (Cytoplasmic isoform) (Usp19 Rat)

Use

Adenoviral

Tags

6XHis-3XFlag

Expression

Mutation

Promoter

CMV

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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rNL1(46-638)

Plasmid

#20172

Depositor

Axel Brunger

Article

Arac et al Neuron. 2007 Dec 20. 56(6):992-1003.

Insert

Neuroligin 1 (Nlgn1 Rat)

Use

Tags

C-terminal 6X His tag

Expression

Insect

Mutation

no splice sites

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pLenti CMV GSE T329A Hygro (w189-4)

Plasmid

#22255

Depositor

Eric Campeau

Insert

Genetic suppressor element #22, T329A

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Threonine 329 is mutated to Alanine (p53 nomencat…

Promoter

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S129A, S133A, S137A

Plasmid

#32456

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S129A, S133A, S137A (S3V, G96R, and P108L also pr…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
mTagGFP2-Cx43-7

Plasmid

#56414

Purpose

Localization: Gap Junctions, Excitation: 483, Emission: 506

Depositor

Michael Davidson

Insert

Cx43

Use

Tags

mTagGFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pDC183

Plasmid

#106415

Purpose

Co-expresses trans SNARE complex with pQZ210 in E. coli

Depositor

Axel Brunger

Article

Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

Inserts

SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)

Use

Tags

Expression

Bacterial

Mutation

Fragment 141-204, Codon optimization and Fragment…

Promoter

T7

Available since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Mef2D-shRNA

Plasmid

#32900

Depositor

Michael Greenberg

Article

Flavell et al Science. 2006 Feb 17;311(5763):1008-12.

Insert

Mef2d (Mef2d Rat)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1-RNA

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pLL3.7 shRNA against CRTC1

Plasmid

#72710

Purpose

Lentiviral expression of shRNA against CRTC1 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC1

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pLAMP1-miRFP718nano

Plasmid

#195749

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

LAMP1-miRFP718nano

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18b

Plasmid

#135554

Purpose

Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18b (Stxbp2 Rat)

Use

Tags

6xHis tag and TEV site

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PKA-RIIβ-sREACh

Plasmid

#114113

Purpose

Mammalian expression of rat PKA regulatory subunit IIβ C-terminally tagged by sREACh.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

PKA regulatory subunit RII beta tagged by sREACh (low-irradiant YFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Rat, Mouse)
TdTomato

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UbC

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 wt

Plasmid

#80885

Purpose

mammalian expression of ALK7 WT

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Use

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
mEmerald-RatLC3B-C-7

Plasmid

#54147

Purpose

Localization: Microtubule-Binding Protein, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

Insert

RatLC3B

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
PB-Gata2

Plasmid

#60665

Purpose

PiggyBac expression vector encoding rat Gata2 downstream of a doxycycline inducible promoter

Depositor

James Thomson

Article

Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

Insert

Gata2 (Gata2 Rat)

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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TSC2-S1345D

Plasmid

#38107

Depositor

Kunliang Guan

Insert

tsc2 (Tsc2 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

S1345D (equivalent to S1389D in rat TSC2 isoform …

Promoter

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only