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pGEX6P-1-SF2

Plasmid

#99020

Purpose

Bacterial expression of SF2

Depositor

Honglin Chen

Article

Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

Insert

SF2 (SRSF1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDA095

Plasmid

#131391

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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RCAS-K-ras

Plasmid

#11549

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (Kras Mouse)

Use

Retroviral

Tags

Expression

Mutation

G12D

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pCJR01

Plasmid

#187888

Purpose

Escherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.

Depositor

Gaylen Uhlich

Article

Uhlich et al Biotechniques. 2022 Apr 13. doi: 10.2144

Insert

DNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS-myrAkt

Plasmid

#11547

Depositor

Sandra Orsulic

Article

Orsulic et al Cancer Cell. 2002 Feb . 1(1):53-62.

Insert

Protein Kinase B (Akt1 Mouse)

Use

Retroviral

Tags

HA tag and myr signal

Expression

Mutation

Promoter

Available since

April 5, 2006

Availability

Academic Institutions and Nonprofits only
pETM33_PEX14_Pex14

Plasmid

#178484

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PEX14_Pex14 (PEX14 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pmGFP-Sec16S

Plasmid

#15775

Depositor

Benjamin Glick

Article

Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

Insert

Sec16S (SEC16B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Nov. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pMALp2x-SCO5461

Plasmid

#42507

Depositor

Koji Okamoto

Article

Nakano et al Toxicon. 2012 Dec 2;63C:55-63. doi: 10.1

Insert

guanosine ADP-ribosyltransferase (SCO5461 Streptomyces coelicolor A3(2))

Use

Tags

maltose-binding protein

Expression

Bacterial

Mutation

Promoter

tac

Available since

April 22, 2013

Availability

Academic Institutions and Nonprofits only
ME2/pBABE-puro

Plasmid

#49164

Purpose

For expression of Malic Enzyme 2 in mammalian cells from retroviral vector

Depositor

Xiaolu Yang

Article

Jiang et al Nature. 2013 Jan 31;493(7434):689-93. do

Insert

Malic Enzyme 2 (ME2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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p414GPD-3xFLAG-Nop4 WT

Plasmid

#169261

Purpose

S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 WT

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

Nop4 WT (NOP4 Budding Yeast)

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

GPD

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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YEpURA-CUP1-HBTubiquitin

Plasmid

#26900

Depositor

Peter Kaiser

Article

Tagwerker et al Mol Cell Proteomics. 2006 Apr . 5(4):737

Insert

HBT-tagged ubiquitin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pET303-ColE1-T-E192C

Plasmid

#180233

Purpose

Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

Colicin E1

Use

Tags

6x His-tag

Expression

Bacterial

Mutation

Truncation of ColE1 containing T domains (residue…

Promoter

T7

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_ZYMND11_coiled-coil MYND

Plasmid

#178493

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_SIN3A_PAH

Plasmid

#178486

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

SIN3A_PAH (SIN3A Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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HpMscS

Plasmid

#78310

Purpose

H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

Depositor

Douglas Rees

Article

Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

Insert

Mechanosensitive channel of small conductance

Use

Tags

6x His Tag and FLAG Tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTH33 [pmyo-3::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168169

Purpose

Expression of YFP-BeCyclOp(A-2x) in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Use

Tags

YFP

Expression

Worm

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pETM6_Ptrc_BsMdh_BmHps_BmPhi

Plasmid

#129104

Purpose

Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus

Depositor

Eleftherios Terry Papoutsakis

Article

Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10

Insert

BsMDH BmHPS BmPHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA4/TO-CDC25A

Plasmid

#216511

Purpose

Expresses CDC25A in human cells under a tetracycline/doxycycline-inducible promoter

Depositor

John Diffley

Article

Zeng et al Cell. 2023 Feb 2;186(3):528-542.e14. doi

Insert

CDC25A (CDC25A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge