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Plasmid#140385PurposeExpresses MphR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-MphR-T
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
LC201: pMVP/PB/Puro-DEST
Plasmid#121874PurposepMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassetteDepositorTypeEmpty backboneUsePiggybac transposon, pmvp gateway destination vec…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-Bi-β-chain
Plasmid#133731PurposeExpresses the gePSI-β-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.DepositorInsertsb-Chain_gePSI
ZsGreen1
UseTagsExpressionMammalianMutationOnly one part of the gePSI-system (startcodon + a…PromoterpTRE3G-BiAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DepoPETase
Plasmid#218702PurposeEncodes codon optimized DepoPETase enzyme for bacterial expressionDepositorInsertDepoPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNJP
Plasmid#115494PurposeExpression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cellsDepositorUseTagsiRFP, mCherry, and mKOExpressionMammalianMutationmCherry S227F, mCherry G229R, mKO V211GPromoterCAGAvailable sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Bxb1-LP-TC
Plasmid#183754PurposeAAVS1 targeting construct with the Bxb1 landing pad cassette.DepositorInsertloxP-eGFP-attP-Bsd-lox257
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE 80L Kan T7WT
Plasmid#102790PurposeExpresses T7 RNA polymeraseDepositorInsertT7 RNA polymerase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 3, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
TFORF3550
Plasmid#145026PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.DepositorInsertmCherry
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-centrin-1
Plasmid#72641PurposeThe plasmid encodes centrin-1 with an N-terminal EGFP tagDepositorInsertcentrin-1 (CETN1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2016AvailabilityAcademic Institutions and Nonprofits only