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Plasmid#138107PurposeGolden Gate recipient and Gateway entry vector; assembly of 3 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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pENTR221 UBR5 ∆PABC
Plasmid#81064PurposeGateway entry vector encoding full length UBR5 Y2402A/K2415A (∆PABC)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆PABC (UBR5 Human)
UseCloningTagsExpressionMutationY2402A and K2415A (bp t7204g, a7205c, t7206c, a72…PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2R3a-TagRFP-OcsT
Plasmid#71270PurposeEntry clone containing TagRFP. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertTagRFP
UseGatewayTags4xGly linker and octaline synthase terminatorExpressionMutationPromoterAvailable sinceDec. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-MCS-LexA-VP16-MiniWhite
Plasmid#165898PurposePuromycin resistant LexA driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
hFLT1 delta CTD
Plasmid#86036Purposehuman VEGFR1 with deletion of the entire intracellular domainDepositorInserthuman Flt1 delta CTD (FLT1 Human)
UseTagsFLAG and HAExpressionMammalianMutationdeta CTDPromoterCMVAvailable sinceApril 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHSG1C3
Plasmid#164423Purposesingle guide RNA (sgRNA) and Prime editing guide RNA (pegRNA) cloning and mammalian cell expression. BbsI cloning for sgRNAs and BbsI/PstI for pegRNAs.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup98-pDONR221
Plasmid#124241PurposeGateway entry clone of Drosophila Nup98-96 cDNA.DepositorInsertDrosophila Nup98-96 (Nup98-96 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pDONR221-Ctdnep1_Cter
Plasmid#196522PurposeEntry vector for Gateway with Ctdnep1_CterDepositorInsertCtdnep1-Cter (CTDNEP1 Human)
UseTagsExpressionBacterialMutationNo transmembrane domainPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR201-Ctdnep1
Plasmid#196520PurposeEntry vector for Gateway with Ctdnep1DepositorInsertCtdnep1-N1 (CTDNEP1 Human)
UseTagsExpressionBacterialMutationPromoterCMV promoter; T7 promoterAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only