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Plasmid#87554PurposeEncodes Boolean Look-up Table logic in response to Cre, Flp, PhiC31, Vika, B3, and Bxb1 recombinasesDepositorInsertEGFP
UseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX330_VCL sgRNA / hSpCas9
Plasmid#172837PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BsCas12a-2AeGFP
Plasmid#123633PurposeMammalian expression, Genome editingDepositorInsertBsCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_SpCas9_USP7 Exon 3 gRNA
Plasmid#131257PurposepX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7DepositorInserthumanised S. pyogenes Cas9 nuclease
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Trex2-bpA
Plasmid#86984PurposeExpression of mouse Trex2DepositorInsertmouse Trex2 coding region (Trex2 Mouse)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, A
Plasmid#107795PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCAS9n(BB)-2A-puro DNM1, B
Plasmid#107796PurposegRNA vector for knock-in gene editing at human DNM1 locusDepositorInsertgRNA targeting human DNM1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX330_rActb sgRNA / hSpCas9
Plasmid#172833PurposeMammalian expression of a sgRNA targeting the intron 1of rat Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of rActb under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Myc-gamma3 (in pCAGGS)
Plasmid#118042PurposeGABAA receptor expression (gamma3 subunit) Myc-tag near N-terminusDepositorInsertGABRG3 (Gabrg3 Rat)
UseTagscMyc epitope EQKLISEEDL inserted between fourth a…ExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9–mKate2ps–EgRNA
Plasmid#62716PurposeEmpty; no U6 gRNA cassetteDepositorInsertCas9–mKate2ps
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330_Tuba1b sgRNA / hSpCas9
Plasmid#172835PurposeMammalian expression of a sgRNA targeting the intron 1 of Tuba1b (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 1 of Tuba1b under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-PrCas12a-2AeGFP
Plasmid#123636PurposeMammalian expression, Genome editingDepositorInsertPrCas12a
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330_rSyn1 sgRNA / hSpCas9
Plasmid#172840PurposeMammalian expression of a sgRNA targeting the intron 21 (last intron) of rSyn1 (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.DepositorInsertsgRNA targeting intron 21 of rSyn1 under the U6 promotor and hSpCas9 under the CAG promotor
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-501V2-FLAG
Plasmid#177096Purpose501V2 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein 501V2 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-BsCas12b-2AeGFP
Plasmid#121948PurposeMammalian expression, Genome editingDepositorInsertBsCas12b
UseTags3xHA tagExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-HDAC4 C-terminus
Plasmid#71400PurposeCAG promoter, mammalian expressionDepositorInsertHDAC4 C-terminus (HDAC4 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-eSpCas9
Plasmid#133370PurposePiggybac vector for constitutive expressionDepositorInserteSpCas9
UseTagsFlagExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-S-B.1.617.1-FLAG
Plasmid#177098PurposeB.1.617.1 variant S-protein with c-terminal FLAG tagDepositorInsertS-protein B.1.617.1 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFREE2-eSpCas9-sgRNA
Plasmid#179580PurposeExpresses eSpCas9 and gRNA that targets ColE1 plasmids for curingDepositorInserteSpCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-B-838;+80pCalb2
Plasmid#66750Purposeluciferase reporter for Calb2 promoter (-838 to +80, WT)DepositorInsertCalb2 promoter (-838bp +80) (CALB2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterCalb2Available sinceJan. 19, 2016AvailabilityAcademic Institutions and Nonprofits only