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Plasmid#165867PurposeTerminal pBlueScript derived high-copy GB Cloning vector with both Alpha and Omega Entry points requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only
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pBlastR-GMRWhite
Plasmid#165885PurposeBlasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165888PurposePuromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165889PurposeBlasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD 1-147
Plasmid#165819Purpose1-147 AA of GAL4 DNA binding domain, requires assembly with TL2-TL4 linker and TL5 activation domainDepositorInsertGal4BD 1-147
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAL4AD 768-881
Plasmid#165820Purpose768-881 AA of GAL4 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domainDepositorInsertGAL4AD 768-881
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMR-WhiteA2
Plasmid#165874PurposeBasic transgenesis 5xGMR white CDS physical eye marker cassette in Alpha2, lacks attB siteDepositorInsertEye Marker Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU2T2U2T2-YFP
Plasmid#84550PurposeHybrid reporter construct with 8 transcription factor binding sites (2 UAS, 2 TetO, 2 UAS, 2 TetO) driving EYFPDepositorInsertU2T2U2T2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT2U2T2U2-YFP
Plasmid#84552PurposeHybrid reporter construct with 8 transcription factor binding sites (2 TetO, 2 UAS, 2 TetO, 2 UAS) driving EYFPDepositorInsertT2U2T2U2-EYFP
UseTagsExpressionMammalianMutationPromoterCMV-minAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
Rap-MESA-20-Tev
Plasmid#84555PurposeMESA protease chain with rapamycin FKBP ectodomain, 20 extracellular linkers, and Tev proteaseDepositorInsertRap-MESA-FKBP-20-Tev
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSM002
Plasmid#122936PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML043C (Marked)
UseTagsExpressionYeastMutationYML043C: bp 52-63 AAGTTAAAGTAT to AAATTGAAATACPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM001
Plasmid#122935PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEC005
Plasmid#122939PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML071C (Marked)
UseTagsExpressionYeastMutationYML071C: bp 52-63 AAACGGTTAAGT to AAGCGCTTGAGCPromoter[tetO2]4inPgal1Available sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_207
Plasmid#122938PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML070W (Marked)
UseTagsExpressionYeastMutationYML070W: bp 52-63 GGGTTTGCCCTT to GGCTTCGCGCTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_243
Plasmid#122940PurposeExpression of the marked mRNA under the control of insertional promoters.DepositorInsertYML029W (Marked)
UseTagsExpressionYeastMutationYML029W: bp 52-63 GAGATTGATCTT to GAAATAGACCTAPromoterPyml029w-tetO(2)Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSM004
Plasmid#122937PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYML065W (Marked)
UseTagsExpressionYeastMutationYML065W: bp 52-63 GAGCAGGGAAAT to GAACAAGGTAACPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_228
Plasmid#122933PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR025C (Marked)
UseTagsExpressionYeastMutationYBR025C: bp 52-63 GGTAATAACTTG to GGAAACAATTTAPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSV_229
Plasmid#122934PurposeExpression of the marked mRNA under the control of substitutional promoters.DepositorInsertYBR026C (Marked)
UseTagsExpressionYeastMutationYBR026C: bp 52-63 AAGCACTTCAAA to AAACATTTTAAGPromoter[tetO2]4inPgal1Available sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-IVS-Syn21-GFP-P10
Plasmid#104615PurposeExpresses GFP upon binding of TALE4 to VAS4DepositorInsert3xVAS4
UseTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only