Addgene

Showing: 1221 - 1240 of 2484 results

Results per page:

Sort results by:

pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1puro-shWillin-B

Plasmid

#40886

Depositor

Paul Reynolds

Article

Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

Insert

shWillin-B (FRMD6 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1puro-shWillin-A

Plasmid

#40885

Depositor

Paul Reynolds

Article

Angus et al Oncogene. 2012 Jan 12;31(2):238-50. doi:

Insert

shWillin-A (FRMD6 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 5, 2012

Availability

Academic Institutions and Nonprofits only
pSpCas9-2A-Puro-RAB7A-gRNA1 (PX459)

Plasmid

#221551

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1A-Puro-mCherry-RAB7-S72A

Plasmid

#221556

Purpose

Expresses Cherry-tagged human Rab7 with S72A mutation in mammalian cells. Compatible with piggybac transposase for genome integration.

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

Rab7A (RAB7A Human)

Use

Piggybac

Tags

mCherry

Expression

Mammalian

Mutation

Change serine 72 to alanine

Promoter

EF1a

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9-2A-Puro-RAB7A-gRNA2 (PX459)

Plasmid

#221552

Purpose

Expresses Cas9 and gRNA for disruption of Rab7A gene in human cells

Depositor

Shawn Ferguson

Article

Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

Insert

gRNA targeting human Rab7A (RAB7A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_puro-mKate-lox2272

Plasmid

#162076

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_hygro-tagBFP-lox5171

Plasmid

#162070

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_blast-tagBFP-lox5171

Plasmid

#162072

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_hygro-tagBFP-lox2272

Plasmid

#162078

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and hygromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_puro-mKate-lox5171

Plasmid

#162077

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable mKate2 reporter and puromycin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_sgRNA_blast-tagBFP-lox2272

Plasmid

#162071

Purpose

3rd generation lentivirus sgRNA delivery backbone with Cre-removable tagBFP reporter and blasticidin resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESh-GFPII-SM-Dlk1

Plasmid

#108932

Purpose

GFP-tagged vector expressing full length isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-Beatrix

Plasmid

#167528

Purpose

Dual-color fluorescent reporter for the creation and detection of Cre-dependent tunable mosaics in floxed mouse lines and cells.

Depositor

Gian Michele Ratto

Article

Trovato et al Nat Commun. 2020 Dec 3;11(1):6194. doi:

Insert

Beatrix

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX_EFS-Cre_ppt-del

Plasmid

#162073

Purpose

Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Insert

Cre

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EFS (part of insert)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_Cas9-blast_GFP

Plasmid

#162074

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and blast resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-blasticidin S deaminase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_Cas9-hygro_GFP

Plasmid

#162075

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-hygromycin B phosphotransferase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESh-GFPII-MB-Dlk1

Plasmid

#108930

Purpose

GFP-tagged vector expressing membrane-bound Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

lacks coding sequence for the juxtamembrane motif…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRESh-GFPII-S-Dlk1

Plasmid

#108931

Purpose

GFP-tagged vector expressing secreted isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Use

Tags

GFPII

Expression

Mammalian

Mutation

contains sequences from full length Dlk1A up to t…

Promoter

Available since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pZP222_INTACT_AT

Plasmid

#225992

Purpose

To make INTACT lines under desired promoter

Depositor

Imran Siddiqi

Type

Empty backbone

Use

Tags

INTACT

Expression

Plant

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge